Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 602 results for: she

Showing: 201 - 220 of 602 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131) or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed...Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012), and counterstained with DAPI. New...the payload sequence into the genome. The team established that they could reprogram the genomic target ...combine for dynamic protein labeling By Alyssa Shepard To fully understand how genes are regulated, it's...promising solution. Recently, the labs of Gord Fishell and colleagues characterized a series of new enhancer-AAV...Leyva Garcia, B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to...

  2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination...to a preregistration, but usually with a fully fleshed out introduction section) to one of 222 participating...scientific research. These include policies for publishers and funders in the Transparency and Openness ...citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/0000-0002...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors...oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016). The protein capsid encapsulating...Manuel Gonçalves' lab found in a second paper published in Nature Methods the same year that delivering...1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr (2016) Viral vectors for gene...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art NIR FPs ...Shcherbakova et al., 2018) and iGECI calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric...daria_gift_shch.     References and resources References Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. How to ...scales. Nat Commun 11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha...near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A. et al. A near-infrared genetically encoded...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... substantial overlap in the activation curves (Marshel et al., 2019) for each tool, and you may have to...the right information When using a previously published system in your own work, you’ll need to know (... Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L. A., Hyun, M.,...13(3), 387–392. https://doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S.,...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... to replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...the monomeric version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric...of plasmid multimers (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101...10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344. Fishel RA, James AA, Kolodner R. recA-independent general...2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number plasmids...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...with your blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers...include recommended blocking conditions on the spec sheet. If you’re still seeing high background after following...longer incubations at both temperatures if needed. Washes Check your antibody manufacturer for wash recommendations...consider adding a detergent like Tween-20 to your washes — although this can be harsh and inhibit some detection... Inefficient blocking Check antibody spec sheet for buffer recommendations Use a chemical blocking...signaling after each round of stripping* Use a harsher stripping buffer Too little signal Large ...

  8. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before You Work ...eyewash station, safety shower, fire blanket, and extinguisher are also required to be present in the room....station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...chemicals, first review their material safety data sheets (MSDS). While You Work Dispose of sharps in a designated...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

  9. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...yearly planning meetings revolve around two simple worksheets, each of which is filled out separately by the...the trainee and PI before the meeting. These worksheets, summarized briefly below (and available of in...facilitate honest conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional...often give ourselves credit for what we have accomplished. Starting with a recap of accomplishments, from...

  10. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...wanted to do when I grew up until I was all but finished my undergraduate degree (and I hope I’m not alone...towards a career in science communication, an established field of inquiry with which I was not yet familiar... a scientific background and bring with them established interests and areas of expertise. Perhaps the...strongest aspect of science communication, then, is its sheer versatility in the face of disparate spheres of ...

  12. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...available. With new fluorescent proteins being published every month, deciding on the best protein for ... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...common use (particularly not the most recently published ones), they can be a good starting point. In many...abundant proteins or marking compartments. I hope this sheds some light on multicolor imaging with fluorescent...

  14. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various serotypes available ...by the small molecule clozapine N-oxide (CNO) (Krashes et al., 2011). Viruses expressing pAAV-hSyn-DIO-hM4D...

  15. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientific communication needs. Going to present some unpublished results at a conference? Post your working manuscript...scientific community. Most preprints are eventually published in journals after formal peer review, with the... as the source or were deposited when the now-published article was a preprint. Depositing plasmids that...bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019) A proposal for the future of scientific...

  16. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...antibody, 0.9–1.1 mg/mL SimplyBlue SafeStain, Thermo Fisher, LC6060 4-12% NuPage Novex bis-tris mini gel, 1mm... LC Area column for the IgG standards in Google Sheets. Open exported CSV file. Select import. Choose ... Chart . This will add a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type ...Add a linear trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart...equation and the R 2 of the trendline in Google Sheets as follows: In the Customize tab, select the Label...

  17. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...affinity: stronger binding antibodies will require harsher stripping to remove, so start with the weaker binding... membrane will remove some of your sample —and harsher stripping means more sample loss. If you are still...a recipe like the ones shared by Bio-Techne, ThermoFisher, or Millipore Sigma. We’ll share the basic composition...solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems offer...

  18. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... easily applied to multiple dishes, remember that handling multiple dishes will take longer and may increase...scaling up by either increasing the number of 10 cm2 dishes or increasing the vessel size. Opting for a larger... opt for simply increasing the number of 10 cm2 dishes. While this option is attractive because the same...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and...

  20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...enhancers, check out the Appendix. Conclusion: Shedding light on dark matter Precise knowledge of which...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...
Showing: 201 - 220 of 602 results