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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1 - 10 of 10 results

  1. CRISPR Guide

    Type
    Guide
    ...platform, termed SHERLOCK, can identify target sequences using a fluorescent reporter. In SHERLOCK, a quenched... the presence of that specific target. SHERLOCK (and SHERLOCKv2) allows for greater amplification and ...bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang lab , is a Cas13a-based...flow strips. Other Cas enzymes can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman, E., Younger, S. T., & Rinn...

  2. Molecular Biology Reference

    Type
    Guide
    ...The insert is typically the genetic element one wishes to study using a particular plasmid. Promoter Region...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic...with a different fluorophore so they can be distinguished from one another. Similar to DNA replication...

  3. Optogenetics Guide

    Type
    Guide
    ... Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and... Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L., Theobald, N., Choi, U...

  5. Antibody Guide

    Type
    Guide
    ...the number of signaling molecules that can be distinguished in your application. The indirect detection ...Cross-link the samples with formaldehyde or UV light. Shear the samples using sonication to break DNA up into...protein. Micrococcal nuclease digestion is used to shear the DNA in these assays. This method is suitable...specific to your antibody and assay may already be published. If it is not, you’ll need to validate the antibody...

  6. Adenovirus Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...remain the most popular choice due to their well-established methods, relative simplicity of production, and...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ..., and VP3, which together form the outer capsid shell that protects the viral genome. Helper plasmid E4...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...

  9. Chemogenetics Guide

    Type
    Guide
    ...for development into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs...

  10. Lentiviral Vector Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...
Showing: 1 - 10 of 10 results