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We narrowed to 323 results for: virus

Showing: 201 - 220 of 323 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...

  5. Recombinases AAV Preps

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    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  6. AAV Molecular Tools

    Type
    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...helper plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming ...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that...outbreaks of food poisoning, the difference is virulence plasmids! This category includes genes that help...instance, vectors derived from the Agrobacterium Ti virulence plasmid are now used routinely in biotechnology...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... of insect cell expression systems include that virus production can be quite time consuming and that ...expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems are.... coli or yeast cells. The only problem with baculovirus systems is that the infected host cell is eventually...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells...

  11. Caltech Systemic Capsids

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    Collection
    ...tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Text...

  12. Common Injection Routes in Mice

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    Blog Post
    ...including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic...

  13. Hot Plasmids February 2024

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    Blog Post
    ...lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive ...

  15. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ... consider including more background on how your virus of interest causes cancer, as well as therapeutic.... She studies the interaction between Human Papillomavirus and the innate immune system, and is interested...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both...improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies...vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector with N-terminal GST pGCS-N5...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome Modifications...

  17. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...other prime editors use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the...

  18. Promega Plasmid Collection

    Type
    Collection
    ... exploring viral biology without disturbing the virus of interest and monitoring endogenous protein dynamics...

  19. Chemogenetics AAV Preps

    Type
    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  20. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector for gene therapy of human diseases... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral...the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV does not integrate into...
Showing: 201 - 220 of 323 results