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We narrowed to 324 results for: virus

Showing: 201 - 220 of 324 results
  1. Viral Vectors 101: Calcium Sensors

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    ...ready-to-use AAV preparations are labeled with the green virus particle on our biosensors page. Whether you’re...
  2. To Each HIS Own

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    ...histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks...
  3. Plasmids 101: Protein Expression

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    ... of insect cell expression systems include that virus production can be quite time consuming and that ...expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems are.... coli or yeast cells. The only problem with baculovirus systems is that the infected host cell is eventually...
  4. Plasmids 101: The Wide World of Natural Plasmids

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    ...helper plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming ...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that...outbreaks of food poisoning, the difference is virulence plasmids! This category includes genes that help...instance, vectors derived from the Agrobacterium Ti virulence plasmid are now used routinely in biotechnology...
  5. Hot Plasmids - October 2020

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    ...Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells...
  6. E11 Bio PRISM Collection

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    ... also be used in conjunction with Cre-enhancer viruses for highly focused circuit mapping and reconstruction...
  7. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ... consider including more background on how your virus of interest causes cancer, as well as therapeutic.... She studies the interaction between Human Papillomavirus and the innate immune system, and is interested...
  8. Common Injection Routes in Mice

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    ...including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic...
  9. Hot Plasmids February 2024

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    ...lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive ...
  10. Caltech Systemic Capsids

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    ...tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Text...
  11. Recombinases AAV Preps

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    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...
  12. New Tools Enable CRISPRa for Neuroscience Applications

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    ...relatively laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...
  13. The AAVantages of AAV in CRISPR Screens

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    ...Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector for gene therapy of human diseases... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral...the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV does not integrate into...
Showing: 201 - 220 of 324 results