...histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks...

...binds its own mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native...

... epitope of the haemagglutinin of the influenza virus, introduced to the field of fusion proteins more...

...transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D...delivered into the brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for...

...which host proteins interact with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding...

... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...

...screen identifies genes essential for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673...

... expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both...improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies...vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector with N-terminal GST pGCS-N5...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome Modifications...

...helper plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming ...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that...outbreaks of food poisoning, the difference is virulence plasmids! This category includes genes that help...instance, vectors derived from the Agrobacterium Ti virulence plasmid are now used routinely in biotechnology...

...Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells...

... of insect cell expression systems include that virus production can be quite time consuming and that ...expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems are.... coli or yeast cells. The only problem with baculovirus systems is that the infected host cell is eventually...

...has systems to defend against both DNA and RNA viruses - 2 cas systems regulate at the DNA level and 1...

...other prime editors use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the...

...for openly shared communication: Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic...

... consider including more background on how your virus of interest causes cancer, as well as therapeutic.... She studies the interaction between Human Papillomavirus and the innate immune system, and is interested...

...it is also possible to follow the life cycle of viruses and intracellular bacteria – have a look at our...

...lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive ...

... Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...