Multiplex Genome Editing with CRISPR-Cpf1
Type
Blog Post
... the virus. Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV
Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector. Lentiviruses carry a (+) strand RNA copy of the DNA sequence...