We narrowed to 873 results for: cel

...physiological intracellular processes with genetically encoded fluorescent biosensors. Subcellular Localization...Kits Allen Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection...Localization Visualize organelles and subcellular structures with well-characterized marker proteins. In Vivo...activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use...activate or inhibit engineered receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent...

... location of your protein of interest within the cell, you will need to tag it with something that makes...fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization... and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for... inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes... Siouxsie Wiles 106476 pET28a_T5-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106473 pET28a_T7-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106474 pET28a_T7-ARG2 Bacterial cells Ultrasound (acoustic reporter gene, ARG2) Escherichia...

...integrase to insert the viral genome into a host cell. Scientists can take advantage of this to stably...integrate transgenes of interest into a variety of cell types. Wild-type gamma-retroviruses have been engineered...of Large T antigen for creation of immortalized cells. See the Weinberg Lab page for additional resistance...Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified version...MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV For...tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

...applications of genetic code expansion in cell biology . Nat Rev Mol Cell Biol, 13 (3), 168–182. https://doi....expanding the genetic code in bacteria and mammalian cells. Plasmid...which means they work independently of the host cell's natural genetic machinery. The orthogonal aaRS ...while E. coli pairs are orthogonal in eukaryotic cells. Pairs from methanogens like Methanosarcina barkeri... are orthogonal both in bacteria and eukaryotic cells. Many of these sets have been mutated and screened... Once these plasmids have been introduced in the cell, the ncAA provided in the media can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal...

...suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly..., iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient media...

...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified...

...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...

...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...and foundations, who share in their vision to accelerate scientific discovery that will help create new... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

...important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although...that is essential for regulating the growth of the cell. p53 p53, the most frequently mutated gene in human...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...