We narrowed to 731 results for: Sis;

...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

...the hippocampus. This technology has formed the basis of the Rett syndrome program being pursued at Vico...nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular...

...be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools were ...function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences ...

...content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

...provenance (this is the history of the cell line consisting of records of the origin and propagation of the...Transition Is Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma...

...occasionally beaten to press, as there is so much analysis possible. Most scientists are, however, privileged...requirements and likely will not do so (calling it a “sisyphean task” given the endless types of data). Rather...

...Wilson, C., Doman, J. L., & Liu, D. R. (2019). Analysis and minimization of cellular RNA editing by DNA... E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with improved...

..., Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers...Ørom, U. a. V. (2023). Recent progress in miRNA biogenesis and decay. RNA Biology, 21(1), 36–43. https:/...

...modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning ...using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015. 75-103. PubMed...

...physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles, ...the samurai sword called a katana. Opto-katanin consists of two separate blue light-sensitive systems, ...

... set of mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at.... Thus yeast can be used to study the molecular basis of human diseases that are caused by a specific ...

... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

... transmitted through the integrin, where it is resisted by the ligand embedded in the extracellular environment...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...

... steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery...consultant and coach in Boston, MA. He specializes in assisting research scientists create productive and manageable...

...Nerve-to-cancer transfer of mitochondria during cancer metastasis. Nature, 644 252–262. https://doi.org/10.1038...was greatly diminished, and tumor hypoxia and angiogenesis were reduced. This seemed to be due to the adipose...

...advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors... culture. However, DNMT3A-induced methylation persisted throughout a 100 day experimental period, as this...

...optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods, 9(2), 159...

...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

...be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does... Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient...

... particularly interested in breast cancer and metastasis as well as teaching and mentoring activities....is a Board Certified neurologist and multiple sclerosis specialist, currently President and owner of THiNKBrain...

...allowing scientists to investigate the fundamental basis of both brain function and dysfunction. First, let...fear-and depression-like behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (...

...not so different from having a thesis committee in graduate school. Thesis committee members provide expertise...

...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach to...you can separately detect these markers in your analysis. Lastly, there are fixable dye stains that react...

...protein tags. The basics These tags most often consist of an enzyme that has been mutated to block its...Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://...

...reveals an essential role for neutrophils in leishmaniasis transmitted by sand flies." Science 321.5891... Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins for multiphoton microscopy...

...the complexity of the screen. For example, the analysis becomes easier, the number of cells needed is ...add value if you are addressing a well-defined, hypothesis driven research question. Examples of custom ...

...contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital. Biochemists...Fessenden! James D. Fessenden is currently an Assistant Professor in the Anesthesia Research department...

... carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic to the...to be resistant to the antibiotic. On the second plate, streak out a strain that’s not resistant to the... be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the...Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant strain grows, it’s likely ... carrying them. The presence of an antibiotic resistance gene on a plasmid allows researchers to easily...for the selection of only those bacteria with resistance to that antibiotic - usually conferred by a plasmid... plasmid carrying the antibiotic resistance gene. The following protocol will allow you to make your own...

...not possible without relying on some kind of pre-analysis. At the end of 2014, I began developing software...bioinformaticians: For flexibility in your own analysis, you need access to raw data, the target sites...

... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also like... Restriction...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation... Agarose Gel DNA Ligation Introduction Gel electrophoresis is the standard lab procedure for separating...pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively...protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well combs Voltage ...solidified, place the agarose gel into the gel box (electrophoresis unit). Fill gel box with 1xTAE (or TBE) until... short DNA fragments is polyacrylamide gel electrophoresis (PAGE), which is typically used to separate...

...became automated with the discovery of a heat resistant DNA polymerase from the thermophilic bacterium...several cycles of denaturation, annealing, and synthesis. After the PCR is complete, the product can be...be verified based on size by gel electrophoresis . Protocol Video Watch the protocol video below to learn...temperature step-wise by 1-2°C. The rate of DNA synthesis is ~1-2 kb/min. The extension time can be adjusted...of DNA that we are interested in amplifying for analysis and manipulation. It is essential that the template...cations can also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the ...that are starting points for DNA elongation and synthesis. Taq DNA polymerase: Special DNA polymerase that...

...your restriction digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot...manufacturer's instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction...sequence (and where they will cut), use a sequence analysis program such as Addgene's Sequence Analyzer . ...visualize the results of your digest, conduct gel electrophoresis . Tips and FAQ Restriction enzymes MUST be ...Dam or Dcm methylase positive strains will be resistant to cleavage at certain restriction sites. See ...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

...separated by denaturing polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody...Run SDS-PAGE and stain gel Day 1 or later: Image analysis Video Watch this instructional video to learn ...pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking platform Fume ...the bottom of the gel. Place the gel in the electrophoresis chamber and secure it. Note: Check the manufacturer...make quantifying the gel easier. Cover the electrophoresis chamber and attach to a power supply. Run the.... Turn off the power supply and unplug the electrophoresis chamber. Remove the gel from the chamber. Use...Addgene QC and would not be used. Section 3: Image Analysis Use ImageJ (Link opens in a new window) or a similar...

...imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay...in appropriate volume of cold lysis buffer. Pro-Tips The volume of lysis buffer will vary depending on...will generally be between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location...the bands. Tips and Troubleshooting The optimal lysis buffer will vary depending on the sample type and...protein of interest. You may need to try a variety of lysis buffers to find the best for your target. The optimal...

...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction enzymes are naturally...and analyze the resulting fragments by gel electrophoresis . The pattern of the fragments on the gel can...enzyme or enzymes that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer ,...to analyze a restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid...manufacturer's instructions) Gel loading dye Electrophoresis buffer Verifying Total Plasmid Size -OR- Insert... tricks see the Restriction Digest and Gel Electrophoresis pages....

... for Ampicillin resistance but is much more important for other antibiotic resistances. Plate some or ...bacterial origin of replication and an antibiotic resistance gene for use as a selectable marker in bacteria... the bacteria time to generate the antibiotic resistance proteins encoded in the plasmid backbone so that...Shorten or skip the outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth, for... plate containing the correct antibiotic. The resistance gene on your plasmid must match the antibiotic...

... Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis is the standard laboratory procedure... once using a multichannel pipette and avoid inconsistent pipetting with our simple tips Bacterial Transformations...visualization and purification. Agarose Gel Electrophoresis Protocol Purifying DNA from an Agarose Gel ...DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel...