...Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock...

...how will you use these systems in your research? Cary Valley contributed to updating this article. References...

... manual droplet generator, you will have to be careful about transferring your droplets from the droplet...

...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which...

...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...

...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...

... the outcome of your IF experiment and deserve careful thought and attention.   * There are other methods...

...experiments must be set up, conducted and analysed with care to get reliable information. Although two-hybrid...

... Caroline LaManna...

...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...

...followed bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can...

...may exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements...

...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...

...Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which...

...and muscle contractions (think skeletal muscle, cardiac muscle, and your airways and blood vessels). Spaghetti...

...the themes in the field and to learn what people cared about. I also used reviews to get a list of research...

... clones. The entry clone and destination vector carry different antibiotic resistance markers (indicated...

... The best option in such instances is palliative care, which helps patients manage the pain and discomfort...

...Wang, Haoyi, et al. "One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated...

...up with new colors (Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability...

...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount...

...it doesn’t eliminate the need to deliver larger cargo such as base editors and prime editors. While using...

...postdoctoral fellow at the University of North Carolina at Chapel Hill. Under the co-mentorship Blossom...

...something fascinating. Behavior is based primarily on careful observation of what an animal does naturally so...

... are many, many different types of experiments carried out by scientists every day. Although the designs...

...and cannot be used for dynamic measurements. Extra care should be taken so as not to destroy the donor molecule...

...typically a tiny fraction of what was actually carried out. While no one is suggesting all data be stored...

...active and on all the time while others are more carefully controlled. Regulated promoters might act only...

... of Molecular Genetics The concept of genes as carriers of phenotypic information was introduced in the...fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal...allowing for selection against any bacteria not carrying the plasmid. Additionally, the restriction enzyme...Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL...µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing...

...Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal... ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a ... to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license or rights...distribution of materials by Addgene is not meant to carry with it, and does not grant any license, express...

...Bacterial Culture Background Information Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...isolate individual (clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture ...Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin...

...away from its usual location, preventing it from carrying out its proper function (Figure 3).  The Hehnly...

... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA...

...fluorescence signal intensity. However, you need to be careful when selecting the primary antibody host species...

...fluorophores, more are in development, including Carrot and Radish from the lab that developed Corn. Aptamers...

...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning...

...phosphoryl-modification; they are both much smaller, and carry only a single negative charge as opposed to a double...

...including dementia, gut disorders, nerve damage, and cardiac disease. This increased disease risk is attributed...

...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...

...three pillars crashes, the lab's success and the careers of its researchers are on the line. With these...

..., the rat is frequently used to study human cardiovascular disease, specifically stroke and hypertension...

... g for 30 sec. Pour off the supernatant, being careful not to disturb the bacterial pellet. Resuspend ...Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents will...the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A...Pour out the supernatant in the sink. Pro-Tip Be careful, the pellet is harder to see and less well attached...

... the western blot. When planning a western, take care to include the proper positive and negative controls...

...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...

...restriction digest and ligation process, you should carefully choose your backbone and insert - these both must...

...Cap the bottle and invert several times to mix. Carefully remove the plate seal from the 96-well plate and...4 °C . Section 3: Primary antibody incubation Carefully remove the plate seal from the 96-well plate and...°C . Section 4: Secondary antibody incubation Carefully remove the plate seal from the 96-well plate and...or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from the 96-well plate and...

...other species VChR1 Channelrhodopsin from Volvox carteri 570 Chronos High-speed, light-sensitive channel...channel from Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2 High-efficiency blue-shifted...Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, et al. 2014. Independent optical excitation...tool for fast neural control derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196...

...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...system is to use a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda...

...protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and limitations...