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We narrowed to 234 results for: Anti-CRISPR

Showing: 221 - 234 of 234 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...with the engineering of fluorescently labelled antibodies made it possible to track cells from one organ...harvesting lymph nodes at different times after antigen challenge, scientists were able to track where ...diverse tissue environments. These studies provide quantitative measurements of cellular motility, interactions...By specifically labeling T cells, B cells, and antigen presenting cells, scientists have been able to ...the bloodstream to invade lymph nodes and scan antigen presenting cells, but two-photon microscopy enabled...scanning was and how fast T cells could jump from one antigen presenting cell to another. Indeed, we now know...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...expression. Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ...

  3. Educational Resources

    Type
    Guide
    ...to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 ...include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness...in-depth instructional content on subjects such as: CRISPR Chemogenetics Optogenetics Genetic Code Expansion...your molecular biology research. Topics include: Antibiotic Concentrations for Bacterial Selection What is...

  4. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral... method detects the expression of viral capsid antigen p24, the FACS dosing method correlates viral titre...reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have been described...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...in regulating translation and inhibiting cellular anti-viral defenses. Glossary Term Definition Adeno-associated...homologous recombination, when both sequences present substantial sequence overlap. Co-expression induces homologous...with trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...

  8. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the...predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location of...

  9. Molecular Biology Reference

    Type
    Guide
    ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for...would not survive antibiotic treatment. It is important to distinguish that the antibiotic resistance gene... grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist...information relating to antibiotic resistance genes as well as additional antibiotics not listed in the table...to confirm which antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock...of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...

  10. Adenovirus Guide

    Type
    Guide
    ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...are also being used in cancer therapy to stimulate anti-tumor immune response in different ways, such as...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...specialized immune cells and antibody production that develops after exposure to an antigen. AdEasier-1 cells BJ5183...vectors are excellent vectors for delivering target antigens to mammalian hosts and have been used as vaccines... individuals within a population that present antibodies in their blood specific to a given virus or infectious...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...concurrently to minimize the impact of neutralizing antibodies developed in response to the first exposure to...AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will be the capsid ...efficiency, and is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1...hormones or metal ions, scientists have adapted an antibiotic resistance operon from E. coli : the tetracycline...#85972 for hygromycin selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid...-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...AIH1, ALGN, AMG, AMGL, AMGX AMH anti-Mullerian hormone MIF, MIS AMHR2 anti-Mullerian hormone receptor, type... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene... ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I AMHR2 anti-Mullerian hormone receptor, type II AMHR, MISR2,...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...

  14. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...
Showing: 221 - 234 of 234 results