Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 627 results for: PROC

Showing: 221 - 240 of 627 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ... toxin which can affect a variety of cellular processes and an antitoxin that suppresses the toxin’s activity...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...need fewer resources for the entire translation process. For example, during high growth rate conditions...segregate with the cell pellet during purification procedures. Even if you produce a large amount of protein...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...transfection, transfection reagent, or transfection process) may have on the target cells. One transfection... the transfection reagents or the transfection process itself has any cytotoxic effects on the target ... optimization of the transfection reagents and process. It is important to note that optimal experimental...

  4. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... for entry of the antibodies. Here, a two-step process is employed: the cells are first fixed and then...phosphatases, and ubiquitin ligases; and many more. This process, however, leaves the nucleus intact. If markers...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...heterogeneous cell population, for example from a processed piece of tissue, where cells are present that ...structural complexity. After several steps of tissue processing prior to running my flow analysis, I can however...interest). Let’s double-click inside our gate and proceed to the next plot! Pro tip! If you only get very...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...body, integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling...investigate the role of specific integrins in cellular processes.   Figure 1: Integrins can shape into three...things IPI, from the scientists and scientific process behind the scenes to the new antibodies and research...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella...could be applied to study other actin-dependent processes in cells and tissues. And, this Opto-RhoGEF design...the protein of interest supports a variety of processes in different cell types. One great way to do this...

  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...gear, and walking everyone through the recording process was going to be time consuming and difficult. Finding...in the future, but we were finally refining the process down to a place I could be happy with it. Cycle...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...These biotin dependent enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid...energy transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule...biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific...

  10. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...that can arise during the plasmid purification process. One is the possibility of damaging the plasmid...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...contaminants can sneak in from almost any step of the process and include things like genomic DNA, RNA, proteins...

  11. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective in bacteria, CRISPR-induced...collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases have connections...properties of CRISPR-Cas systems is base editing. This process is generally accomplished by fusing an enzyme capable...

  12. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...steps in the viral production and quality control process to improve both the yield and quality of the viral...scientists with the means to study biological processes on the cellular level instead of having to work...

  13. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    .... Listen to this episode for insights into the process of getting an MD PhD, the inside scoop on developing...family. 3:56 - 7:04 : Alex describes the MD PhD process and explains why he loved the first 2 years of ...

  14. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...experiments - and we've updated our submission process so you can easily add your data, too!   Using...should!) submit your data to the Data Hub! The process is easy with our new submission form - and no worries...

  15. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...antibody worked in your application. The submission process is easy, with simple, descriptive fields, drop-..., and can be saved at any point throughout the process, so you can work through it at your own pace. To...

  16. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Improvements that can be made to the grad school process 22:41 - END : Niroshi and Ben's favorite advice...Future of Research Looks to Improve the Research Process  Browse our Career Posts Resources on Addgene....

  17. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...scientific sharing. So we set out to streamline this process. We take pride in facilitating the exchange of ... and by whom. For Recipients: Streamlined Processing: When you place an order, Addgene generates the...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    ...of optogenetic control of endogenous cellular processes." The LITE system can now be applied to unraveling...genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids have clear...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... be expensive and time-consuming to repeat the process with these new photoconvertible proteins. With ...state after excitation at 561 nm. This imaging process worked well with the common GFP variants EGFP, ...
Showing: 221 - 240 of 627 results