We narrowed to 556 results for: SEC

..., and nanobody. For more information read our secondary nanobodies blog post.  A rapid selection platform...RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View...

...target sequence for U6 promoter expression and the second site accepts the homology arm repair template. ...fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology arms and an N- ...

... reads should align to this reference sequence. Second, we perform a megaBLAST search on all the reads...vectors for your research. Please use the comments section below to let us know if you have any specific questions...

...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012. Additional Resources on the Addgene...

...summer of 2014 (after extensive discussions with biosecurity experts, ecologists, and national authorities... Holm, D. G. & Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement...

... pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression...

...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

...plants, microbes, blood or mammalian cells in 30 seconds or less without using a pipette.  Here’s how this... from plants, animals and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal....

...topic. For example, it may be useful to adapt the second version (multiple presenter, figure by figure) ... seemed to be of use, it’s worth scheduling the second and other subsequent meetings. The number of participants...

...to say “no, that’s not in my wheelhouse”, take a second to reflect. For example, say your boss approaches...Maybe you also showed critical thinking skills by dissecting their problem and piecing together a really great...

...necessary to create a replication-competent virus. Second, Tat is eliminated through the addition of a chimeric...us know how you use lentivirus in the comments section below! References 1. In vivo gene delivery and...

...-like endonuclease domain, but they lack Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in a ...to cleave again and possibly mediate HDR. This “second chance” mechanism might improve the frequency of...

...is transferred to a 2D barcoded tube (see next section) and every time something is removed from this ...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

...fluorescent reporter, the brain tissue was extracted, sectioned, and imaged, with all image sets available at ...information and includes annotated images of the brain sections describing the labeled regions, as well as the...

...long-read technologies. Short-read sequencing (“Second-generation sequencing”)  Short-read sequencing ... or complex sequences such as G-C rich areas, secondary structures, or repetitive regions. Short-read ...

...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...

...shared server. The blue box labeled “A5” on the second rack in the corner freezer sounds easy to remember... some things I could pay attention to, so my dissections go smoother next time?” or more generally “How...

...expression to appreciable levels. We wanted to create second-generation mARGs with higher expression, a smaller... structural gene gvpA marked with EBFP2 and the second cassette contains the P2A-linked accessory genes...

... date with their third party libraries and any security updates. Most of the time, updating these libraries... which means there will be no further updates, security or otherwise. This might sound like Greek to you...

...quantifying AAV: qPCR efficiency can be affected by second-order structure of the AAV genome, caused by repeats...2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which is at...