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We narrowed to 1,045 results for: STI;

Showing: 221 - 240 of 1045 results
  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...
  2. Fluorescent Protein Guide

    Type
    Collection
    ...Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid Collection New England...
  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...cap: institutions of higher education nonprofit entities related to or affiliated with institutions of ...Having a lead role or critical role at a prestigious institution: This criterion is consistently under scrutiny...exempt from the cap if the applicant works at institutions of higher education, a related or affiliated...would not have been possible. Display of work in artistic exhibitions or showcases (not usually applicable...Proactivity is very important in this case.  You can still assist your PI in the peer review process, but remember...to self-petition. You need the support of your institute to do this one. Universities will usually only...professors. Casseus Law has seen that research institutions are willing to sponsor postdoctoral researchers...
  4. DNA Purification Without a Kit

    Type
    Blog Post
    ... placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally...requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying tendency... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...commercial kits, but in less time. The authors estimate that a gravity-based column plasmid maxiprep kit...slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices...solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass particles...detergent, and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic...
  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...Recent studies carried out by Sebastian Lourido and colleagues constitute an important step toward understanding...Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed an alternative...publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized...bacteria, the process of cloning, combining, and testing these different parts can be quite arduous. The...your gene of interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning...
  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...ordering food - everything is covered.   So why are we still printing and gluing pieces of paper in notebooks... needs The familiar paper-based lab notebook is still a standard approach to record-keeping in many labs...In their ideal form, ELNs provide users with a holistic means to manage their work, combining data, team...researchers. The full story is available here. Our questions focused on researchers’ current practices, their...maintenance and support.” Besides that, academic institutions tend to think about the long term, so they need... once they establish the system within their institution. In the study, respondents were also asked about... knowledge of electronic lab notebooks into 9 questions that will help you start and define what exactly...
  7. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... order.  Still have questions? Visit the Addgene Help Center If you have additional questions about our... my order? All of these questions are really getting at one bigger question: How does a customer place...answers to other ordering questions here. In addition to answers to common questions about the ordering process... discussing some of our most frequently asked questions related to the ordering process: I placed an... purchase order, which is an official document listing materials and pricing information that is sent ...repository. We distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents...
  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits —...recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV...researchers' favorite features and limitations of existing backbones, we're excited to share our very own... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic... therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a...-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with...added to the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened...
  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... a function of digestion time. For phage DNA that is refractory to enzymatic digestion, however, a physical...diverse biological entities on our planet, but we still know collectively little about how they infect and...RBPs, the host recognition factors of phages) constitute one of the most popular classes of phage proteins... the process of capitalizing on phage RBPs as diagnostic tools and therapeutics. In addition, the strategic...recognize on a host cell. To make matters even more interesting, much of what a phage faces as it approaches ...library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately gene-sized...units, which can be purchased cheaply, are small plastic containers that can easily be attached with a hose...
  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge in cell biology...at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, ...contributed by the gene editing team at the Allen Institute for Cell Science. Learn more by visiting the Allen...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...genomic tagging experiments. Browse the Allen Institute for Cell Science Plasmid Collection Fluorescent...edited hiPS cell lines generated using the Allen Institute for Cell Science Plasmid Collection, please visit...Many thanks to our guest bloggers from the Allen Institute for Cell Science. This post was contributed by...
  11. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... do during your training is meet interesting people doing interesting things. I call this building relationships...will be asking the questions, so be prepared. There are many good lists of questions on the internet. To...learning new things from other scientists doing interesting work. Consider this to be like any other research...mind that this is a good time to ask personal questions about salary, opportunities for advancement, challenges...challenges and disappointments. These more personal questions can elicit the most valuable insights you will...people like to help One good place to look for interesting connections is your university’s alumni database...too busy to respond to you). You will uncover interesting opportunities by meeting with people working ...
  12. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...last week.  This fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica...oft quoted statistic that only 15% of current postdocs will be academic principal investigators. She also...for science—we have powerful tools, beautiful questions, and smart scientists.  At the same time, he pointed...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...action Each of the speakers in the panel had interesting words of experience and encouragement.  Dr. Sibby... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award...
Showing: 221 - 240 of 1045 results