We narrowed to 324 results for: cloning

...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...

...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled...

... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...

...Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...their concentration. However, for most standard cloning (where the insert is smaller than the vector) a... is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark for average...

...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...