We narrowed to 326 results for: cloning

...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...

...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...and SLC binder sequences in Gateway-compatible cloning vectors (pDONR, pENTR), bacterial expression vectors...NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning References Frommelt, F., Ladurner, R., Goldmann...

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled...

... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...

...Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...their concentration. However, for most standard cloning (where the insert is smaller than the vector) a... is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark for average...