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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 326 results for: cloning

Showing: 221 - 240 of 326 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

  2. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone... Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

  6. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

  7. Ras Pathway

    Type
    Collection
    ...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background...

  8. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  9. Distribution to Industry

    Type
    Collection
    ... Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic...

  10. A Look at Addgene's QC Process

    Type
    Blog Post
    ... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids...

  11. dTAG - You're it!

    Type
    Blog Post
    ... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...

  15. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...their concentration. However, for most standard cloning (where the insert is smaller than the vector) a... is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark for average...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...

  17. Worm Expression Resources

    Type
    Collection
    ...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...
Showing: 221 - 240 of 326 results