We narrowed to 332 results for: cloning
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TypeBlog Post...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...
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Addgene: The First Twenty Years
TypeBlog Post...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector... -
Resolute Plasmid Collection
TypeCollection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025... -
CRISPR Plasmids - C. elegans
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...their concentration. However, for most standard cloning (where the insert is smaller than the vector) a... is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark for average... -
Mammalian RNAi Tools
TypeCollection...Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone... Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral... -
dTAG - You're it!
TypeBlog Post... additionally provides detailed information on cloning procedures used to construct the deposited plasmids... -
A Look at Addgene's QC Process
TypeBlog Post... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target... -
Genome-wide Screening Using CRISPR
TypeBlog Post...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ... -
Synthetic Biology - Assembly Standards Guide
TypeCollection...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common... -
Ras Pathway
TypeCollection...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background... -
CRISPR Plasmids - Drosophila
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...proteins and empty backbones for expression and cloning of fusion proteins, along with key parameters to...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow...Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD...mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression pFA6a-link-yomCherry-SpHis5... -
10 Basic tips for mammalian cell culture
TypeBlog Post...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...Type: Choose an empty backbone if you intend to clone your own insert, or use the other options to find... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post... primer Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...