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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 242 results for: gRNA

Showing: 221 - 240 of 242 results

  1. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...Veening is now available with one sgRNA per operon or gene. The sgRNA pool can also target core operons... cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...operons in other pneumococcal strains. The BARBEKO sgRNA library uses CRISPR cytosine base editors to disrupt...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...indicating loci where SNPs reside on sgRNA target sequences for sgRNAs that target ancestry-associated genes...that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral bias....

  3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that ...modules are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a ...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    ...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  11. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...primers flanking each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine...complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see step 1.4 ), 1.0 μl sgRNA 24- or 25-mer reverse...composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following...delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a...posted at the top of the page. CRISPR Design Design sgRNAs manually or using freely available online tools...non-coding elements. For creating a gene knockout, two sgRNA located within exons will enrich even monoallelic...
Showing: 221 - 240 of 242 results