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We narrowed to 9 results for: gRNA

Showing: 1 - 9 of 9 results

  1. Plan Your Experiment

    Type
    Guide
    ...how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...the associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

  2. CRISPR Guide

    Type
    Guide
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...if the gRNA spacer sequence shares sufficient homology with the target DNA. Once the Cas9-gRNA complex...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting...library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library... screen. The figure above is an outline of how a gRNA library is used. Below are the general steps to ...experimental and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism...

  4. Lentiviral Vector Guide

    Type
    Guide
    ... require gRNAs for each target. Lentiviral vectors are a common way of delivering these gRNAs, due to ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...

  5. Addgene's eBook Collection

    Type
    Guide
    ...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens...

  6. Adenovirus Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle...

  7. Promoters

    Type
    Guide
    ...also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ... Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV capsid is challenging due ...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...
Showing: 1 - 9 of 9 results