... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...interactions in live Drosophila. eLife 7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola...Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006... living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201 Kerppola TK (2013) Bimolecular...Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G...Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117 Kodama Y, Hu C-D (2012) Bimolecular...update and future perspectives. Biotech 53. https://doi.org/10.2144/000113943 Additional resources on the Addgene...

... priority and have organized many events over the years. To keep the organizational side of things from...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play... in getting the fun started. I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes...posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure... her operational responsibilities, she enjoys organizing team building events at Addgene and cultivating...

...screens. bioRxiv 2024.03.17.585235; doi: https://doi.org/10.1101/2024.03.17.585235.   Improved prime editor...RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038/s41586-024-07259-6. PMID: 38570691. Antibodies...monoclonal antibody. Virology. 558, 28–37. https://doi.org/10.1016/j.virol.2021.01.003. PMID: 33714753. ...emitting at 525 nm. Image reused from https://www.janelia.org/open-science/voltron-and-positron.  Both...2023) Neuron, 111(10), 1547–1563.e9. doi: https://doi.org/10.1016/j.neuron.2023.03.009. PMID: 37015225.  ...Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org/10.1101/2023.12.12.571321.  Revolutionizing Gene...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

... Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587-023-01821-9 He, Y., Zhou, X., Chang.... Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N.,...National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X...glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5 Tong, H., Wang, X., ...Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587-022-01595-6 Ye, L., Zhao, D., Li...Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/s41587-023-02050-w Yi, Z., Zhang, X., Wei...glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w Additional Resources...

...proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E., Qin, Y.... Nature Cell Biology, 26(2), 294–304. https://doi.org/10.1038/s41556-023-01342-2 Koblan, L. W., Arbab...Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938-z Koblan, L. W., Doman... Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S...cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587-020-0609-x Yang, C., Dong, X., Ma...Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002/advs.202202957 Yuan, T., Yan, N., Fei, ...methods. Nature Communications, 12(1), 4902. https://doi.org/10.1038/s41467-021-25217-y Zhao, D., Li, J., Li...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...Thomas-Kilmann Conflict Model, courtesy of https://managementweekly.org/thomas-kilmann-conflict-resolution-model...

...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like... about a local food pantry or community arts organization. Many people are surprised to learn that Addgene...nonprofits do with their revenue. A nonprofit organization cannot distribute profits to private individuals...as a nonprofit, we attract partnerships with organizations that share our community-oriented values. Addgenies...Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey...Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our Mission Meet the Addgene...

...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik KC, Wienecke... proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou...neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional resources...controlling gene expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene...

...fluorescent data on all channels - helpful if you forget to set the channel wavelength before you run the...14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson...Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker...Reprogramming Process. Sci Rep 8, 8716 (2018). https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ;... quality data. Sci Rep 7, 2409 (2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; Nadeau, K...7, 2019, Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  ...Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano J. Real-time...

... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium... additional resources and information about organizations promoting reproducibility in this booklet (which...within a published manuscript. Luckily, many organizations have been working to provide tools that make...expand to other protein technologies later. Organizations such as BenchSci and FPbase are working hard... they’ve used for data analysis while other organizations like Dryad make it easier for researchers to...are blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of...editing technology NgAgo). In addition, there are organizations like ReFigure which aims to consolidate published...

... colors you like. Controls and validation Can’t forget the controls! The necessary control experiments...immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough, L., ...Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov...1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H., Antonietti...publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161 McLaughlin, K. (2019...Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov, E....Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 Additional Resources on...

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...Lentiviral Transduction Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After...in developing reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global... would want to know before, during, and after organizing such a workshop. Finally, the group put a lot...IS-MPMI. If you’re interested in having a workshop organized at your upcoming conference or you’d like to join...

...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...Biophysica Acta (BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla...ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987.... Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi... years on. Nature Protocols 1:581–585. https://doi.org/10.1038/nprot.2006.83 Additional resources on the...for purifying DNA without a kit Resources on Addgene.org Check out our molecular biology protocols Find...

... cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer E, ...Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy M, Cohen-Barak E, ...in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO WA, Reisfeld...Monoclonal Antibodies. Hybridoma 7:453–456. https://doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler M, Wacker...quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister... single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380  Additional resources on the...BlottingPlasmids 101: Biotinylation Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids...

...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...activation or repression of gene expression (see www.nursa.org for a curated list of coregulators). Coregulators...context defined by ligand, cell type, and promoter, organizing the expression of functional groups of genes ...Nuclear Receptor Signaling Atlas (NURSA) at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor...

...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...E. coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...

... 179 (2020). https://doi.org/10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi...The nuts and bolts. Methods 116:51–62 . https://doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-Gaita V, ...Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE,...Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang ... Skp. Front Cell Infect Microbiol 3: . https://doi.org/10.3389/fcimb.2013.00072 Additional resources on...