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We narrowed to 666 results for: otos

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Showing: 221 - 240 of 666 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the...target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting...further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in the field, and...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family...RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1 LTB lymphotoxin beta (TNF superfamily...member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1... RNase A family, 2 (liver, eosinophil-derived neurotoxin) EDN, RNS2 ROBO1 roundabout, axon guidance receptor...3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3...family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636, MGC119637 ...MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035, MGC120036, MGC120037...

  7. Brain Initiative Collection

    Type
    Collection
    ...pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination Ofer Yizhar ...

  8. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ...in nontransgenic and cre driver mice. Current protocols in neuroscience / editorial board, Jacqueline ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...

  9. COVID-19 Resources

    Type
    Collection
    .... Other Resources Protocols Coronavirus Method Development Community at Protocols.io (Link opens in a ...new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping...linked to collections of open-access articles, protocols, and other resource collections related to COVID... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Research Articles...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit: This ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

  11. CRISPR Guide

    Type
    Collection
    ...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. https://doi.org/10.1038/nprot...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine...SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers....202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70....2015.08.016. PMID 26365381 (Link opens in a new window) Protocol for using the Improved Reagents (ca. 2015) from....2012.08.031. PMID 22982858 (Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab:...

  14. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Large Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags ...LSSmKate2-C1 - Mammalian Expression Return to top Photoactivatable (Off to On) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Return to top Photoconvertible (e.g. Green to Red) Protein Excitation (nm...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  18. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...

  19. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...
Showing: 221 - 240 of 666 results