We narrowed to 660 results for: otos

... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species...Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium... page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR...

...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the...target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting...further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in the field, and...

...hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family...RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1 LTB lymphotoxin beta (TNF superfamily...member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1... RNase A family, 2 (liver, eosinophil-derived neurotoxin) EDN, RNS2 ROBO1 roundabout, axon guidance receptor...3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3...family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636, MGC119637 ...MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035, MGC120036, MGC120037...

...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ...in nontransgenic and cre driver mice. Current protocols in neuroscience / editorial board, Jacqueline ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...

...pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE...frame with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination Ofer Yizhar ...

.... Other Resources Protocols Coronavirus Method Development Community at Protocols.io (Link opens in a ...new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping...linked to collections of open-access articles, protocols, and other resource collections related to COVID... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Research Articles...

...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

...the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit: This ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli...system has generally low efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune...

...Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine...SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers....202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70....2015.08.016. PMID 26365381 (Link opens in a new window) Protocol for using the Improved Reagents (ca. 2015) from....2012.08.031. PMID 22982858 (Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab:...

...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags ...LSSmKate2-C1 - Mammalian Expression Return to top Photoactivatable (Off to On) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Return to top Photoconvertible (e.g. Green to Red) Protein Excitation (nm...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...

...to create the most informative and entertaining protocol and explainer videos for you to level up in the... This could be you after watching Addgene’s protocol videos. via GIPHY   If you’re also looking...

... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...

...Resources on Addgene.org Check out Our Protocol Pages for New Protocol Videos View Our Molecular Biology Reference...

...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

... plasmids here, and check out our streak plate protocol video on Youtube:     Additional resources...Addgene Check out additional written and video protocols ...

...Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID...to Making AAV? Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from ...

...contains a biosensor that detects both serotonin and serotonergic drugs. Check out these new vectors, and...