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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: otos

Showing: 221 - 240 of 658 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed ...

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...

  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ...in nontransgenic and cre driver mice. Current protocols in neuroscience / editorial board, Jacqueline ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...

  7. Plan Your Experiment

    Type
    Collection
    ...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...used as positive controls when working out a new protocol. Read more about how to design your gRNA . Synthesize...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to...aberrant axon initial segment (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector...expression of transcription factors for sensory, motor, and cortical neuron differentiation. Held et al...opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine...SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers....202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70....2015.08.016. PMID 26365381 (Link opens in a new window) Protocol for using the Improved Reagents (ca. 2015) from....2012.08.031. PMID 22982858 (Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab:...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit: This ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags ...LSSmKate2-C1 - Mammalian Expression Return to top Photoactivatable (Off to On) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Return to top Photoconvertible (e.g. Green to Red) Protein Excitation (nm...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...

  12. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  13. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...

  15. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ... 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory...Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN... at the final assembly step in the Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag ...

  19. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...
Showing: 221 - 240 of 658 results