...in reinforcement learning, decision making, and motor control.  What do we understand about Dopamine?...Learn about Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research...

... the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach...Addgene founder, Melina Fan, recently shared our protocol for making competent cells to save time and money...

...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...

... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies...

... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

... intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation,...reversible and do not cause structural deformation or photo-induced damage. These fusions can also function ...

... blog post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs ...Learn more about Addgene's viral service Find protocols for working with virus ...

...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9...without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes...

...goal is to provide scientists with new tools and protocols that they can easily incorporate into their own...service Learn about viral production Find viral protocols here ...

...recombinant antibodies, educational materials, protocols, and shared data, on our values of transparency... antibody collection.  Addgene is sharing our protocols for recombinant antibody production as well as...

... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

...Database, bas006 (2012). PubMed. For a stepwise protocol on designing and using RNA scaffolds: Delebecque...scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

...consist of a 20-mer (“protospacer sequence”) upstream of an “NGG” sequence (“protospacer adjacent motif” or...This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol for Genomic Deletions in Mammalian Cell Lines ... lines. Along with the video, you can find the protocol section from the original publication below. You...52118 (2015). PubMed Full Access to the Paper & Protocol Video Download the manuscript here: Bauer et al... and genetic elements in mammalian cell lines. Protocol For referenced Tables and Figures, please access...Figure 2A ). NOTE: In this example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom...

...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

... the workshop, the participants took part in a protocol drawing exercise. The workshop also used a shared...different parts of the poster. Conferences, such as Photonics Online Meetup, hosted their poster session on ...

...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...verification to find additional tips and detailed protocols on topics such as how to set up your digests and...Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...