We narrowed to 239 results for: p
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TypeBlog Post...Many thanks to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ...
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CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...glycine tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, cleave them, and... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...Blog Posts Read Our Primer on Optogenetics by Derek P. Simon Read Our Interview with Ed Boyden Resources... -
Deep Dive: qPCR
TypeBlog Post...potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate and statistically verified... -
27 Hot Plasmids from 2016
TypeBlog Post...suitable for high throughput applications. Trepte P, et al. J. Mol. Biol. 2015. PubMed PMID: 26264872 ...Text. Seeliger MA, Young M, Henderso MN, Pellicena P, King DS, Falick AM, and Kuriyan J. Protein Sci. 2005... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...Furthermore, they find that during stress, mRNAs in P-bodies are translationally repressed whereas the nonsequestered... has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput... -
CRISPR Guide
TypeGuide... Schaepe, J., Du, P. P., Lotfy, P., Bassik, M. C., Bintu, L., Bhatt, A. S., & Hsu, P. D. (2022). Systematic... 29556040 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson...target DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA pegRNA P rime E dit...Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li,...Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa, A., Rekhtman,...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin.... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C.... -
Chemogenetics Guide
TypeGuide...Link opens in a new window) Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015). The First...PMID: 25587888 (Link opens in a new window) Coward P, Wada HG, Falk MS, Chan SD, Meng F, et al. (1998)....Lee H-M, Sciaky N, Simmons A, Nonneman RJ, Huang X-P, Hufeisen SJ, Guettier J-M, Moy SS, Wess J, Caron ..., Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals...Takuwa H, Hori Y, Oyama K, Ji B, Takahashi M, Huang X-P, Slocum ST, DiBerto JF, Xiong Y, Urushihata T, Hirabayashi... -
Modular Cloning Guide
TypeGuide...for protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast...MoClo-YTK toolkit for protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast...Sauer 62 plasmids for advanced strain engineering in P. pastoris (a.k.a. K. phaffii ), including overexpression... CRISPR/Cas9 mediated genome editing in the yeast P. pastoris (a.k.a. K. phaffii ). NT-CRISPR Plasmid ... -
Plan Your Experiment
TypeGuide...27984732 Doman, J. L., Sousa, A. A., Randolph, P. B., Chen, P. J., & Liu, D. R. (2022). Designing and executing...CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system that enables...Dixit, A., Parnas, O., Li, B., Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D., ...Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, ... -
Lentiviral Vector Guide
TypeGuide...Hinrichs, C., Highfill, S. L., Somerville, R. P., Panch, S. R., Jin, P., & Stroncek, D. F. (2022). Genome-wide....2016.17 PMID: 27110581 Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M....Sabatini, D. M., Chen, I. S., Hahn, W. C., Sharp, P. A., Weinberg, R. A., & Novina, C. D. (2003). Lentivirus-delivered...12649500 Timms, R. T., Tchasovnikarova, I. A., & Lehner, P. J. (2018). Differential viral accessibility (DIVA... -
Adenovirus Guide
TypeGuide...new window) Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L., & Gao, G. (2021). Viral vector platforms within...Custers, J., Vellinga, J., Hendriks, J., Jahrling, P., Roederer, M., Goudsmit, J., Koup, R., & Sullivan...new window) Mendonça, S. A., Lorincz, R., Boucher, P., & Curiel, D. T. (2021). Adenoviral vector vaccine...in a new window) Rosewell, A., Vetrini, F., & Ng, P. (2011). Helper-dependent adenoviral vectors . Journal... -
Adeno-associated virus (AAV) Guide
TypeGuide...Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. J., & Muzyczka, N. (...T., Inaba, T., Mizukami, H., Ozawa, K., & Colosi, P. (2004). The adenovirus E1A and E1B19K genes provide...Link opens in a new window) McCarty, D. M., Monahan, P. E., & Samulski, R. J. (2001). Self-complementary ... -
Optogenetics Guide
TypeGuide...22866029 Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Wiegert JS. 2015. An improved chloride-conducting...PMID 26965121 Yizhar O, Fenno L, Zhang F, Hegemann P, Diesseroth K. 2011. Microbial opsins: a family of...Beyrière F, Tsunoda SP, Mattis J, Yizhar O, Hegemann P, Deisseroth K. 2008. Red-shifted optogenetic excitation... -
Science Guides
TypeGuide...Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems, which form an... -
Sequencing Primers
TypeGuide... in pRS vectors Pry1 CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer pTrcHis Forward GAGGTATATATTAATGTATCG... -
Immunology Research Plasmids and Resources
TypeCollection... 2 J2, TCRGJ2 TRGJP T cell receptor gamma joining P JP, TCRGJP TRGJP1 T cell receptor gamma joining P1... External Resouces Immport : Bhattacharya S, Dunn P, Thomas CG, Smith B, Schaefer H, Chen J, Hu Z, Zalocusky... -
Molecular Biology Reference
TypeGuide...Met M AUG Phenylalanine Phe F UUU, UUC Proline Pro P CCU, CCC, CCA, CCG Serine Ser S UCU, UCC, UCA, UCG... -
Neurodegeneration Plasmid Collection
TypeCollection...tac ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA] SNCA U6 Parkinson's Edward Burton 75637...