We narrowed to 564 results for: pac

...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive ...Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells allows production...and composition of your library can drastically impact what conditions it needs to properly amplify.  ...

...  Here's what you'll find in this post:  New compact, efficient prime editors A versatile and precise...tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna Stroik The ...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983–4002...

...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

...Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent ...” gate in the beginning should not have a great impact on your analysis, and I often choose a looser gate...separation pattern largely stays the same and does not impact my ability to draw a proper gate, I am free to ...

... professional capacity generated during this process result directly in positive impacts on the lives ... a level of sympathy that is difficult to find. Impact of the International Mentorship Program and the...

... and infect specific cells. This protein shell packages the vector’s genetic material, and variations ...serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2 ✓ ✓ AAV2-retro...Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request Browse all in-stock AAV preps Browse...

...techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ from the short-read...1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer reads are able to... Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production. Biotechnol...

...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...

... to using plasmid pooled libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....

...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...

... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using a new UNG...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Adeno-associated...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids...

...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...

...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...

... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...

...titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic response from...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...