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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 562 results for: pac

Showing: 221 - 240 of 562 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent ...” gate in the beginning should not have a great impact on your analysis, and I often choose a looser gate...separation pattern largely stays the same and does not impact my ability to draw a proper gate, I am free to ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more. Metal Ions Target Description...Dynamics in the Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. ACS Chem Biol. 2018 Jul 20...levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ from the short-read...1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer reads are able to... Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production. Biotechnol...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Adeno-associated...Addgene has an extensive collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA ...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E....E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (4 6 ) combinations...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using a new UNG...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 ...transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the...

  15. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

  16. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...

  17. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic response from...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...

  18. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and he tweets...

  20. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...Running your transfer in the cold room or packing the box with ice packs can help keep the system cool, as can...overheating. Try running it in a cold room or putting ice packs in and/or around the gel box. If overheating remains...
Showing: 221 - 240 of 562 results