Viral Vectors 101: Producing Your rAAV
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Blog Post
... our Viral Vectors 101 series!
This post was originally written by Didem Goz Ayturk and updated by Ina...
We narrowed to 303 results for: rigi
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Polymerase Chain Reaction Overview and Applications
TypeBlog Post...statistics to determine the concentration of the original template DNA in the sample. Addgene currently... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...identify AAV-44632, the contaminant we mixed into the original AAV sample at 5%. Detecting 5% contamination is... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...of protein fusions or expressed independently) originally fluoresce green or blue and, when they mature... -
10 Basic tips for mammalian cell culture
TypeBlog Post...ensures that you have many cells stored in their original condition and can replace your working stock if... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...generated info for your own purposes, or delete the originals if desired. It works as a whole or in part. For... -
Pooled Library Amplifications
TypeBlog Post... Addgene strives to distribute aliquots of the original library whenever possible. In this blog, we’ll... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain the most popular deposited... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...was first pioneered by Voie and colleagues and originally named orthogonal-plane fluorescence optical sectioning... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post... of two proteins from a single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt Genomics Blog and is republished... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Although the 2nd generation system is safer than the original system, the possibility of creating a replication-competent... -
CRISPR Antimicrobials
TypeBlog Post...systems. Phagemids are plasmids containing a phage origin of replication that can be packaged into replication-incompetent... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was not included amongst... -
Getting to Know Expansion Microscopy
TypeBlog Post... evenly in all directions to several times its original size. Depending on the protocol, samples can be... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...proteins of interest has occurred. Although the original Y2H systems utilized the yeast Gal4 activator,... -
Plasmids 101: Protein tags
TypeBlog Post...combinations still include at least one element from the original TAP tag. The terms dual-labeling and dual-tagging... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...