We narrowed to 526 results for: sequence

...The raw data is then normalized to a reference sequence present in the same amount in all samples (usually...to run multiplex assays, allowing the reference sequence and the gene of interest to be amplified in the...for both the gene of interest and the reference sequence. At the end of the run, each droplet is read as...Complexity of the gene of interest Some gene sequences are harder to amplify and quantify than others...

... generating insertions or deletions in the DNA sequence. This same approach is effective to target short...very small deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed...Cas9 generates double-strand breaks, a new DNA sequence can be knocked-in, or introduced, into the break...off-target effects since promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions...

...generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are...

...use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately, the vast majority... majority of antibodies are supplied with no sequence information, leading to problems with reproducibility...affinity reagent screening, and next-generation sequencing of hybridomas have opened the door for a new ...

... editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the...complex, promoting its interaction with the degron sequence and ultimately its degradation. While other eukaryotes...

...sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013). Modifying the CRISPRi system... will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional regulator...Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152...

...accessibility in research and therefore makes the sequences of these affinity reagents available to scientists...

...promoter, enhancer, or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse...

... a genetic sequence and integrate it into any DNA sequence containing the 38-50 bp sequence recognized...they optimized the specific attachment sites via sequence engineering, enabling even more efficient integration...

...please contact us. Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

... effective, ivTRT may be more challenging for sequences with complex secondary structure. Today, multiple.... Miura et al. (2018) suggest using a 50-80 bp sequence around the desired insertion site to search for...C-terminal insertion with Easi-CRISPR. Potential guide sequences (arrows) and their PAM (bold highlighted text)... knock-in design may naturally remove the gRNA sequence or place it far from the PAM; if not, you can ...guide. This design also separates the gRNA target sequences from their PAMs, preventing re-cutting after editing...

... a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent between plants...contain a plant pol III promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos...is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several...the specifics - promoters used, precise protein sequences or domains, and methods of delivery - the underlying...

...the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF. Negative...protein is present, but cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein... (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In the presence...binds to promoters containing putative operator sequences (CuO) (linked in groups of 6), allowing transcription...which activate their respective UAS and lexAop sequences but are also regulated by QS and quinic acid. ...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system...

...repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used ...plant source), by tuning the length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies...

...protocol before performing single-cell RNA sequencing. The sequencing results covered TFs representing 92% ...average of 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced cellular...

...transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery... genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4...multiple genes under the control of UAS enhancer sequences. For example, if you discovered five new genes...

... (bEBPs) at a sequence upstream of the promoter called the upstream activating sequence (UAS). The Wang...phenotype can be identified by next-generation sequencing of the genomic DNA. However, some cell types ...

.... Piecing together multiple PCR products using sequence homology is also common. Overlap extension PCR... to splice together two PCR amplicons based on sequence homology, and the subsequent product is then digested...fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria...

...inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon on pTargetF (Jiang... this system, you’d first introduce your sgRNA sequence into the pTargetF construct and then introduce... process begins with the addition of the sgRNA sequence in pEcgRNA. Next both plasmids are transformed...pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ccdB gene containing BsaI...