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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 221 - 240 of 538 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    .... Piecing together multiple PCR products using sequence homology is also common. Overlap extension PCR... to splice together two PCR amplicons based on sequence homology, and the subsequent product is then digested...fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...longer stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. ...variants with optimal function. High-throughput DNA sequencing is then used to measure the frequency of each...more codons you test in one library, the more sequencing reads you will need. See Fowler et al for more...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... strand at each replication fork. Termination sequences present in the chromosomal DNA ensure that the...pSC101 itself. RepA binds to directly repeated sequences called iterons located near the ori and directly...ColIb-P9 plasmid, is complementary to part of the sequence forming this pseudoknot, so its binding prevents...replication may differ in the specialized proteins or sequences necessary for initiation or the mechanisms that...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...platform is compatible with enrichment, single-cell sequencing, and optical pooled screens in human cells. Addgene... improve the specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence...alone or in combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia... REVeRT to functionally reconstituting coding sequences for a range of applications, including CRISPR ...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon on pTargetF (Jiang... this system, you’d first introduce your sgRNA sequence into the pTargetF construct and then introduce... process begins with the addition of the sgRNA sequence in pEcgRNA. Next both plasmids are transformed...pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ccdB gene containing BsaI...

  6. CRISPR Plasmids - Yeast

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

  7. All Antibodies

    Type
    Collection
    ...details including the protein and plasmid sequence, the antigen sequence, and cross-species reactivity. Reactivity... trusted partner labs or is predicted based on sequence homology with the antigen. Addgene supplies a ...

  8. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...DNA prep, the plasmid sequence has been validated by next generation sequencing. The DNA concentration...Quality Control Plasmid verified by next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid...

  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...working on lab operations software for next-gen sequencing (NGS), and high-throughput screening. While some...in the US to learn where the gaps are in their sequencing workflows, any problems they’re having with our...region. She is particularly interested in DNA sequencing tech, and the intersection of technology, software...

  10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...in your organism or cell type? You can use DNA sequencing or other molecular cloning techniques to determine...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame.... A gRNA that targets your desired gene (whose sequence is also found in the reporter gene). A control...

  11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half of the base... cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond at the split...markers, transformation acceptor vectors, and sequences for genomic integration by homology directed repair...

  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...: shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting...

  14. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...These vectors contain an Autonomously Replicating Sequence (ARS) derived from the yeast chromosome. As the...part of an ARS along with part of a centromere sequence (CEN). These vectors replicate as though they ...DNA (apparently independent of a known specific sequence) dictate the replication of these vectors. S. ...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Use the target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-...recombinases and tyrosine recombinases, based on sequence similarity and the amino acid residue used in ...DNA strand (in cis) in the same direction, the sequence between the sites is excised as a circular piece...recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...target sites. Codon optimization: Modifying the DNA sequence of the recombinase to align with your organism's...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...HHCS-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss...however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed from their...

  17. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...lowering Cas9 expression, and truncating gRNA sequences used for targeting; however, these options can...the gRNA (see figure below) Because off-target sequences have less complementarity to the gRNA, they have...Lab similarly thought that if they could weaken sequence independent interactions between Cas9 and DNA,...

  18. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... available, and the collection of samples and sequencing is being organized and funded by someone else...the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline. It’s possible... the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and the reagents...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ...transcription initiation when targeted to DNA sequences within core promoter regions. pTAL5-BB contains... place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into ...visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome Visualization...white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression in mammalian...vectors for use in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for...

  20. Five Popular Model Organisms

    Type
    Blog Post
    ...also the first eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation. ...organisms, the C.elegans genome is also completely sequenced and over 60% of human genes have an ortholog in...Kerstin, et al. "The zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446...extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959-3970. PubMed...
Showing: 221 - 240 of 538 results