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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 210 results for: FAST

Showing: 121 - 130 of 210 results

  1. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCCCTCCAATGGCAGCAaagcccttaagcttcaATTCGGGCTTATTAATCA fastkd3 TAL3438 & TAL3439 TACCTGTCTTCTAATGAGgaccacgcttttatgaAACGCCTCTCCTCTTGTA...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    .... This method of cell lineage conversion can be faster, bypasses intermediate pluripotent stages, and ...

  4. AAV Production in HEK293 Cells

    Type
    Protocol
    ...of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave or...

  5. CRISPR Library Amplification

    Type
    Protocol
    ... that contain a recombinant plasmid likely grow faster in the closed system of a crowded plate or culture...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

  7. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

  8. The time and cost required to make a plasmid

    Type
    Blog Post
    ...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  10. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... viral DNA for quality control. This gives us a faster turnaround time than short-read sequencing, as ...The workflow converts the raw sequencing data to FASTQ format, cleans and normalizes reads using the BBTools...program called Geneious. Within Geneious, the raw FASTQ reads are used for either guided or new de novo ...
Showing: 121 - 130 of 210 results