...: they require switching between a two-ring purine and one-ring pyrimidine base. This is a far more complex... the side groups of DNA bases and leave the core ring structure intact, allowing them to make 4 out of...backbone, making them efficient and precise tools for altering DNA sequences.
These first base editors were both...researchers in the Keith Joung lab, who were engineering an ABE to reduce its tendency to make off-target...sites.
To explore this further, the team started engineering a CBE to be worse at its original job. They ...glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil editing, and instead introduced...GBE editing but not CBE editing, maybe because repairing a GBE-derived abasic site requires more cellular...
