...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays...specific to your assay. Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells...cells. Immunofluorescence experiments often use a nuclei marker to identify individual cells and additional... PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting...

... tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To repeat the knock-in...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted...articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic Acids Res. 2014 The Förstemann lab has provided...

...Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial...TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao...TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging...

...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ...

...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...also requires two unique single stranded DNA oligonucleotide (oligo) primers, which anneal to the regions...and the hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is ...increases the error rate of the DNA polymerase. Deoxynucleoside Triphosphates (dNTPs): Are the building blocks...strands of the template DNA. They are strands of nucleic acid that are starting points for DNA elongation...

...and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third day of CRISPR, Addgene gave...

...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...etc.) involves selecting one or more unique oligonucleotide primers that flank the regions of your plasmid...colored peaks corresponding to one of the four nucleotide bases. This is an example of a trace file from...

...needs. We may even be able to alter the type of nucleic acid sequences that Cas9 can target." As further...

...ssDNA ends from the ssDNA-specific endonuclease activity of T5 Exonuclease and furthermore reduces secondary...specific and unique function for the reaction: T5 Exonuclease - creates single-strand DNA 3’ overhangs by chewing... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments...

...for human genome editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b...

...technology, the ability to direct enzymes – from nucleases to transcription factors – to specific sequences...

...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs...

...matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides. Together...

...standard curve RNase-free DNase 10X DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical...0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid...10 9 stock or previous dilution (μL) Volume of nuclease-free water (uL) Molecules per μL 10 90 2 x 10 ...Dilution Series Volume of sample (uL) Volume of nuclease free water (uL) Dilution factor Total dilution...

...microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...