We narrowed to 556 results for: SEC

...shared server. The blue box labeled “A5” on the second rack in the corner freezer sounds easy to remember... some things I could pay attention to, so my dissections go smoother next time?” or more generally “How...

...fluorescent reporter, the brain tissue was extracted, sectioned, and imaged, with all image sets available at ...information and includes annotated images of the brain sections describing the labeled regions, as well as the...

...long-read technologies. Short-read sequencing (“Second-generation sequencing”)  Short-read sequencing ... or complex sequences such as G-C rich areas, secondary structures, or repetitive regions. Short-read ...

...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...

...expression to appreciable levels. We wanted to create second-generation mARGs with higher expression, a smaller... structural gene gvpA marked with EBFP2 and the second cassette contains the P2A-linked accessory genes...

...quantifying AAV: qPCR efficiency can be affected by second-order structure of the AAV genome, caused by repeats...2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which is at...

...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on its...

...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...

... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...

...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...

...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...

...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...

...of biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who ... I’ll highlight some of these questions in the sections below. Mentoring relationships can help identify...

...several applications of this technology. In each section below, we cite the original paper but link to plasmids...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...

...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...

... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...

... that we describe in the Materials and Methods section of our paper (1). Lastly, it is possible to build...sequencing as described in the Material and methods section of our paper (1). How might one troubleshoot GSRMs...