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We narrowed to 545 results for: SEC

Showing: 241 - 260 of 545 results

  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...

  4. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... date with their third party libraries and any security updates. Most of the time, updating these libraries... which means there will be no further updates, security or otherwise. This might sound like Greek to you...

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...non-optimal codons are associated with specific secondary structures in 10 closely related yeast strains...sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts Effects on transcription...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...quantifying AAV: qPCR efficiency can be affected by second-order structure of the AAV genome, caused by repeats...2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which is at...

  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...

  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...

  17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...
Showing: 241 - 260 of 545 results