We narrowed to 732 results for: SON
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TypeBlog Post... were excited to deliver the very first ones in person by our own Tech Transfer Team! Why MTAs Matter ...Laboratory Dana-Farber Cancer Institute Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering...
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5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...mentoring groups seem to be as successful as in person groups. Maybe it’s just that it’s easier for everyone...A How-To Guide for Scientists. Once in-person, peer-mentoring groups at Addgene have gone virtual... -
Summer SciComm: Science Storytelling
TypeBlog Post...thought about how X works?”, or even by introducing a person that the audience can relate to. It doesn’t need... Rachel Leeson... -
Intro to Our Summer SciComm Series!
TypeBlog Post...communication (it is, after all, how one moves from layperson to expert) and peers can and do consume science... Rachel Leeson... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post... and then applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue...983. https://doi.org/10.1177/002215549804600813 Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral...272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth... -
10 Great Guest Posts We're Thankful For
TypeBlog Post... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...this post, Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...Lederberg's Personal Perspective: Plasmid (1952-1997) Cohen, Stanley N. “DNA Cloning: A Personal View after... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...founding and running miLab to describe some key lessons I learned while becoming a health science entrepreneur...cold email Outside of the potential contacts your personal network can offer, you are really on your own ... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...from science-focused to business-focused?" The reason is that I knew that the mind-set of the employer...instead I thought they would focus on “Has this person been consistent throughout their career?”, "Have... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...Just getting together and chatting with another person about their career path is a great start to a mentoring... read before your meetings and discuss these in person. See the extensive resource list available from... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post... those at the molecular and cellular level. The reason for this is cost and complexity – anyone who works...curriculum to see if any of the activities fit your lesson plans. If you are an instructor at the college ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post.... The problem is…how can you make a “hands-on” lesson on genetic manipulation of human stem cells when...clearer ideas about gene therapy in the following lessons. And let me tell you more, they had brilliant ideas... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...sustained by the availability of this strain from The Jackson Laboratory. Originally developed and characterized...this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has... -
DAP arrays for multiplex gene editing
TypeBlog Post...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716). Fig. 2: Comparison of previous representative dual-gRNA architectures... -
25 Work From Home Tips for Scientists
TypeBlog Post...Taking Care of Your Behavioral Health. Work on personal development Take the myIDP (individual development...a challenging period for everyone for numerous reasons, and you shouldn’t expect yourself to be as productive... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...projects. The idea behind the platform was simple: any person working on COVID-19 could file a request on our...collaborations beyond COVID-19 One year on, our biggest lesson running the platform has been the realization that... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...treatment. These responses vary depending on the person. However, in silico and in vitro assessment of ..., M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz, J., Goletz... -
Cultivating Community Science at BosLab
TypeBlog Post...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage more involvement... -
How to Lead a Great Meeting
TypeBlog Post...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working... -
Advice for Scientists Starting a Lab
TypeBlog Post...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...disengagement. By tailoring my leadership approach to each person, I was able to create a more productive and cohesive...similar transitions can provide you with insights, lessons from their experiences, and practical advice for... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...never picking up the phone you don’t get to know a person. These interactions could either help you in your...an MBA in finance and a B.S. in accounting from Babson College. He is a certified public accountant (CPA... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...inactive state, and cheap. Unless there’s a specific reason you can’t use it for your ELISA and Western, I’... Rachel Leeson... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu ...