We narrowed to 753 results for: ELP

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...of virus with various tropisms.  Packaging using helper-free packaging cell lines This method utilizes ...

2. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ..., as compared to wild type, would be extremely helpful as a complementary approach. Also, in principle...

3. Screening for Successful Genome Editing with Digital PCR

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   ...Thankfully, there are many great resources available to help guide you through the rough waters of mutation validation...

4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...replication-incompetent phage particles with assistance from a ‘helper’ phage prior to transduction. Scientists also use...

5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ... cell type is already known, these reporters can help define the different cells present in a mixed population...

6. Targeted Mutagenesis with EvolvR

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   ...tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate the evolution...

7. 15 Years of Addgene: The Top 15 Plasmids

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   ... viral vector service in 2016...we’re excited to help scientists share their reagents with the scientific...

8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ... homologs. Evolving better base editors with the help of phages! The latest ABE from the Liu lab is ABE7.10...

9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...materials. The use of reporting guidelines can also help remind us to include all of the essential details...

10. Behind the scenes: Addgene’s new search engine and more

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    ...involved). The new homepage and navigational elements help users who prefer to browse and discover items in...

11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...advantages and limitations of each Cas enzyme can help determine which enzyme to use. Remember, accessibility...

12. Viral Vectors 101: Viruses as Biological Tools

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    ... blogs on virus safety and viral applications to help you start (safely!) thinking about the possibilities...

13. Plasmids 101: Screens vs. Selections

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    ...look at an outcome. Your scientific question can help determine which is right for you. Screen variations...

14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...research, modeling patient mutations using SDM can help determine if a variant is causal for a given disease...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...efficient, what other challenges does this system help to solve? Myers: Only a small percentage of the ...

16. Plasmids 101: Blue-white Screening

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    ...brom-indigo). So far, so good, but how does this help us screen plasmids? Blue-white screening in the ...

17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...P65-HSF1 plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide...

18. Plasmids 101: Yeast Vectors

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    ...  Yeast selection marker What does the marker help synthesize? Counterselection? (growth-based positive...

19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...plasmids recently deposited by Chris Voigt’s lab help keep gene expression stable regardless of copy number...

20. dTAG - You're it!

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    ...disrupting protein function. A literature search can help determine which terminus is favorable for tagging...

21. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ... TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression...

22. RANbodies: Reporter Nanobody Fusions

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    ...ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique...

23. Antibodies 101: Affinity Reagents

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    ...Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as the OG (as ...

24. Antibodies 101: Selecting the Right Antibody

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    ...Western blot, a secondary antibody is ideal as it will help to amplify the signal. Some applications use either...

25. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...other short epitope tags (Kimple et al., 2013) could help improve the transport of protein of interest the...

26. Addgene: The First Twenty Years

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    ...was established as an e-commerce company, which helped it stay ahead of the curve as access to the Internet...

27. Antibodies 101: Fab Fragments

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    ...components of your experiment. These considerations will help you narrow down which antibody format to use for...

28. Human Germline Editing Using CRISPR

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    ...gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially in the...

29. CRISPRainbow and Genome Visualization

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    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...

30. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...feedback from all members of the research community to help us develop these resources further. Feel free to...

31. Viral Vectors 101: An Introduction to AAV

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    ...used to ensure specificity; this is particularly helpful when wanting to target specific neuron subtypes...

32. Plasmids 101: The protein expression toolbox

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    ...would like. Check out our promoter blog if you need help choosing. Viral expression is another mechanism ...

33. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...kilobases, but only in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective ...

34. Antibodies 101: Conjugation

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    ...antibodies in your refrigerator often need a little help to be useful. Mainly because, well, antibodies are...

35. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...Gradinaru Labs developed a machine-learning model to help them construct “designer” channelrhodopsins with...

36. Plasmids 101: Golden Gate Cloning

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    ... detailed Golden Gate protocols, complete with helpful tips and tricks, see The Sainsbury Lab website ...

37. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...tons of parts and characterizing the software to help you put it together. The software will be available...

38. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...mutations accumulated over time? Undergraduates can help to answer this question! The Pathways Over Time...

39. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...synthetic decaffeination operon podcast segment With the help of engineered microbes, used coffee grounds could...

40. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ... being the additional amplification step, which helps to visualize low-abundance proteins. Figure...