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Showing: 601 - 650 of 1052 results
  1. Working 9-5: an Addgene Co-op

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    Blog Post
    .... The following Friday, I received the official offer. I was so happy to hear back, I accepted that day... food service industry, which followed a very different schedule. Fortunately, I was provided with a detailed...gave me enough time to become familiar with the different types of issues that were important to this function...Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At this point...
  2. 6 Steps to Submitting a Resume That Gets Seen

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    Blog Post
    ...the future. Studies show that the majority of job offers arise as a result of existing professional (and...interest, even excitement, in a position. Everyone prefers to hire someone that will be excited to be there...resume should also be customized to highlight different skills and to get the important information in..., you will never get the help that can make a difference. Having your resume forwarded personally gives...
  3. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    Blog Post
    ... value, clearly communicate exactly what a site offers to their users, and most importantly, allow those...get scientists excited about what Addgene has to offer, and to help scientists find what they need - whether...wanted to highlight our blog, daily updates, and offer better exploration throughout the site.  Blugene...2011, and while you may have seen our mascot at conferences, on our website, or in social media, we wanted...
  4. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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    Blog Post
    ...plasmids for expressing different types of rhodopsins and sensors as well as references and links to other ...using both its Addgene ID number as well as the reference for its original publication. To help facilitate... citation that you can put straight into your reference section. Why do this? Not only does citing plasmids...
  5. 9 Tips to Achieve Success in Academia

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    Blog Post
    ...eyes to the many different jobs associated with science, especially if the conference is a big multidisciplinary...curious and interdisciplinary.   3. “There are many different ways to succeed – as loner or gregarious;  dictator...leaving academia should present their research at conferences to obtain a “crash-course in all the real life...
  6. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional approach of an autonomously...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these...YGOI on a western blot or under a microscope, we offer a collection of vectors carrying several fusion ..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...
  7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post
    ...compare and analyze any differences between our sequence results and the reference sequences. Using Snapgene...features To align NGS sequencing results with reference sequences To design experiments for further plasmid...Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...
  8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post
    ...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...
  9. SunTag and Fluorescent Imaging

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    Blog Post
    ...nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...
  10. Build Your CRISPR Vocabulary

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    Blog Post
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the spacer...scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins, either a crRNA...
  11. Year of the Camelid: Antibody Style

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    Blog Post
    ...the discoveries yet to come.      References and resources References Arbabi-Ghahroudi M (2017) Camelid...become appealing candidates for the detection of different cancers (Jin, et al. 2023).  So where do all these...    Figure 2: Genetically modified mice offer an alternative method to producing single-domain...
  12. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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    Blog Post
    ....  3. Do you prefer being a generalist? Working on a variety of projects and in different fields of research...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...
  13. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post
    ...plasticware or think about conserving reagents and buffers.  However, I had very environmentally conscious...required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying..., I was bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took... small enough to minimize the required area and buffer volume.  So, I designed my own gel box (see illustration...
  14. Antibodies 101: Epitope Availability

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    Blog Post
    ...outside of the cell. The same protein may have different epitopes available if it is being selected out...controls in validation, as you may need to test different configurations and conditions to understand exactly... what a given protein is doing or changing in different conditions. Just remember to keep good records...when you’re building your antibody library! References and Resources More resources on Addgene’s blog...
  15. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post
    ...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get ...
  16. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post
    ...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound...40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...
  17. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes...viability question. The miLab you see today is far different than what was originally planned, due in large...the potential contacts your personal network can offer, you are really on your own to build a strategic...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible...
  18. Another Pathway into Cells: iTOP

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    Blog Post
    ...specificity of their luciferase assay, they concluded that components in the buffer used for protein purification...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...
  19. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post
    ...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...
  20. What the HEK?

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    Blog Post
    ...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...for and in HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight...
  21. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...
  22. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ... together as useful and productive, forming a different constellation of interests and skills than what... experimental design and data with authors and referees. And I am constantly teaching – often explaining...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...short program to build skills and connections. AAAS offers a summer program that gives graduate students an...
  23. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    Blog Post
    ...Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership was fruitful...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...
  24. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...
  25. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...side of caution when adding buffer back to the solution. Add a bit less buffer than you would to achieve...process of generating viral particles. Limit tube transfer - the fewer opportunities your viral particles...working on methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant...
  26. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ...Sequencing and QC at Addgene.  References and resources References Mosch, R., and Guchelaar, H.-J. (... T-cells or monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through...vitro approaches enables researchers to develop safer and more effective mAb therapeutics. The in silico...
  27. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post
    ...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature...
  28. Finding and Joining Your Dream Lab

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    Blog Post
    ...tend to be more "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing...presents regularly with labs around the world. You may prefer a more competitive style to spur you to succeed..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...
  29. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM...Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...
  30. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ...Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that...giving a public talk, ask a friend who works in a different field to listen to you practice.  Ask them what...addition to this annual event, graduate students in different regional and disciplinary communities gather at...here: https://comscicon.com/. All our events are offered free of charge to accepted students, and we pay...
  31. Clear and Accessible Writing

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    Blog Post
    ...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...
  32. Viral Vectors 101: Viral Applications

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    Blog Post
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even...
  33. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ...consumer-grade components. In this field, often referred to as 'frugal science', existing components are...problem-solving platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper...microscope. Lower left panel shows an image of differentiating monocyte with planes and hardware to it. Right...image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling...
  34. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ...abhiaggarwal.bsky.social‬).   References and Resources References Aggarwal, A, et al. (2025). A sensitive...are used simultaneously to monitor or control different cell types or signals in the brain. For this to... to work, each indicator needs to emit a different color of light and be compatible with the lasers available...
  35. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post
    ...found in genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular...signaling pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are...cancer mutant collection and the genetic pathway reference collections are described in this paper Systematic...Central PMCID: PMC5386591.  The genetic pathway reference collection is described in this publication ...
  36. Antibodies 101: Flow Cytometry Controls

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    Blog Post
    ..., without the FMO, it would be difficult to differentiate the Ly6C negative population from the Ly6C positive...like for a particular marker, you can use the reference plots provided by the antibody companies or check... use the APC IgG2c isotype control). The only difference is that the isotype antibody will not target ... as to when you need isotype controls. Our lab prefers to use them to gate our populations, but others...
  37. Plasmids 101: Yeast Vectors

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    Blog Post
    ...widely utilized across many different cell types and each one requires different elements for vector propagation... features of yeast vectors and explain how they differ from the bacterial elements previously described...auxotrophic markers and includes the associated references.  Yeast selection marker What does the marker...
  38. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...stem cells edited to carry mutant versions of 15 different disease-associated genes. Addgene spoke to Musunuru...mutations. Once that's done in stem cells, we can differentiate those mutant and wild type stem cells into any...all have the mutations but they will also have different genetic backgrounds. It becomes more of an apples-to-oranges...suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system...
  39. RNA Extraction Without A Kit

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    Blog Post
    ...final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...ultracentrifuge.  Figure 1: An outline of the different steps in RNA extraction. Researchers at the... Reagent®, RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... ensure DNA contamination is not an issue.  References                 Allewell NM, Sama A (1974) The...
  40. The Future of Research Symposium Boston 2015

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    Blog Post
    ...of trainees? Are there too few or too many at different levels? What does an ideal and productive workforce...a young scientist’s perspective, and the many different paths that biomedical graduates take in their ...policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing US biomedical...Henry, and Michael Roach. "Science PhD career preferences: levels, changes, and advisor encouragement."...
  41. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type IIS restriction...256 distinct flanking sequences, sequences that differ by only one base may result in unintended ligation...constructs, assemble standard plasmids parts in different combinations, or other new and exciting applications...constructs in a single, high-efficiency step. References: Sequential amplification of cloned DNA as tandem...
  42. Addgene: The First Twenty Years

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    Blog Post
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for...2013, which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...
  43. Antibodies 101: Fab Fragments

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    Blog Post
    ... for your specific scenario.    References and resources References Bates A, Power CA (2019) David vs...is relatively easy to physically divide up the different regions, both through genetic and proteolytic ...broken up, there are a few commonly available different fragments that retain antigen binding (Figure ...
  44. Plasmid Tools for Microbiome Studies

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    Blog Post
    ... microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... Jennifer Tsang...
  45. All in a Twist: dsRNA

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    Blog Post
    ... disease, and much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et ... dsDNA viruses yield dsRNA through a slightly different mechanism thought to be associated with bidirectional...blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...
  46. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post
    ...negative) the action potential of a cell, the more differentiated and quiescent it is, and the more depolarized... toggled between open and closed states using different wavelength light pulses. Other resources from...bioelectricity, bioelectric researchers in many different fields came together to found the journal Bioelectricity...regeneration, medicine, and synthetic bioengineering. References Chernet, Brook T., et al. "Use of genetically...
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