...which CRISPR genome editing system you use, there remains a chance that the observed phenotype was caused...especially the clinic. Popular validation assays are insufficient As discussed in CRISPR 101: Validating Your ...that a single cell clone derived from the pool contains both the rare off target event and the desired...laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge. Outside of the...

...Andel Institute. Krampus was created by Nate Levine.             View this post on Instagram                     ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...

...with Wei Leong Chew, a researcher at the Genome Institute of Singapore who recently started his own lab....work to an independent position at the Genome Institute of Singapore. Chapter 3: Biology research and ...a Junior Principal Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies...

...materials repository contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024...

... You’ll be able to use these GIFs on Twitter, Instagram, TikTok and more. Just type “addgene” into the...looking for that perfect GIF for your TikTok or Instagram story, we also have stickers to help express your...looking for new ways to help you celebrate those wins in the lab.   You don’t need to sweep confetti...

... with “A Better Way to Share Science” for: The inside scoop from scientists who have deposited plasmids...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.  ...

...Purification protocol page for more detailed instructions. AAV titration by qPCR using SYBR green technology...copies in a given AAV preparation. For detailed instructions and calculations on how to prepare a plasmid...study biological processes on the cellular level instead of having to work with a whole organism and can...

...aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested...their CSV file from Addgene by following these instructions, and then uploading the CSV with request data...to the dashboard (Fig. 2).     Figure 2: Insert showing the upload function for the Addgene Requests...

...minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...

...Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate research...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

...plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...

...Feng Zhang (of the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research) – and... the neuroscience community pulls off such an inspiring, thought-provoking, collaborative event – all ...

...      If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds for you as well... might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other ...  Aliyah Weinstein...

... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...target." As further proof of just how big and mainstream the news on CRISPR has gotten, consider these...30. (Pubmed) Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. ...

...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... Find job listings at Addgene Get more career insight from Addgenies ...

...the methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color...

...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...journey since starting Addgene. Thank you for inspiring us with your breakthrough research and your commitment...

... you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette under the control...of these options would be very time consuming. Instead, a quick way to get the results you need would ...complicated ways to use this particular AAV. For instance, you might use it as part of a flex switch you...

... I was able to express my visions for what my Instagram posts would look like. While working remotely ...continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role... Some of recent Meet an Addgenie posts on Instagram! From this project, I observed what it was ...

...the page looked clean, professional, and modern. Instead of trying to place all of the content above the... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ...

...plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...promoter has been modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis...regions permit multiple regulatory elements to be inserted, further customizing gene expression. Sahoo et...

...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award...

...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...when incubated in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human...

...systems. As a part of this effort, members of the Rawlins Lab have compiled a new toolkit for performing ...SL fluorescent biosensors by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control...in tobacco protoplasts, the enzymatic biosensor retains its catalytic activity.  As SLs are also known ...

...activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The...by working with organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...

...favoring the separation of the two helixes. Low pH instead results in the breakage of the ester bonds responsible...the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA negative...mutated to neutralize the nuclease activity. For strains that still carry this gene, there are usually extra...

...is expressed. In cells where the stop cassette remains, the effector is not expressed and the cells are...leads to gene constructs where the stop cassette remains following recombination. This strategy results ... Aliyah Weinstein...

...calculators These links can help you calculate how much insert and backbone you need for different types of cloning...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics ...

...were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J. Cell biology. Fixing...

...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ..., whether within academia or industry. Albert Einstein once said: “There are only two ways to live your...

... I spend much less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists...mean abandoning it or running away screaming, but instead can involve walking alongside it and giving it ...graduate training. PhDs have many roles to play inside, on the outskirts, and entirely outside of academia...

...this is a much-needed community resource. Instead of painstakingly searching for experimental details in ...serotype, promoter, or tool type and each entry contains an image along with key experimental details (...

...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

... to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this person ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...

... of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze samples ...der Geer P, Manning MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat...10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman...

...was robust, capable of generating a wide range of insert sizes and also produced high yields of quality ... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...being overwhelmed by all the new information, and instead allow me to pay attention and answer my key questions...

... became our bacterial transformation guru and instructor for our intro to biohacking courses. Our members...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

...platform to help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus...use this potential to make a difference not just against COVID-19, but also in other areas of scientific...Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL...

...DSB) Cas9 and its variants have two endonuclease domains: the n-terminal RuvC-like nuclease domain and the...conformational change that positions the nuclease domains to cleave opposite strands of the target DNA. Thus...refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide target sequence to direct...

...system less bulky and easier to use with other strains of E. coli, Schmidl et al. condensed the system...ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription factors... optimal. These tools are compatible with many strains of E. coli, as well as with other plasmid-based...

...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers... to an end and your next professional adventure begins. While many career pathways exist for scientists...

...impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease” assays (e.g...then performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time...your genome edits, this paper provides detailed instructions on how to design such assays, in addition to...

...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...research proposal is the perfect fit to their institutional focus and that you're better equipped than anyone...with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to [email protected]. Many...

...at the level of nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes...for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence... Eric J. Perkins...

...interaction between neuroscience and other fields. For instance I met one researcher studying how the microbiome...’ve developed in your current work to make new insights in neuroscience. Even if you're currently a synthetic...select viral vectors in the repository, you can instead have Addgene make the virus for you. Neuroscientists...

... ABEs are only weakly compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing...The latest ABE from the Liu lab is ABE7.10 which contains the deoxyadenosine deaminase, TadA-7.10 (Gaudelli... of naturally occurring enzymes and functional domains speed up the development towards faster and more...

...Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online, searchable...structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information ... More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources in...

...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...well as related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

... to make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids,...Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time it takes...your experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try constructing...

...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through...the importance of analyzing sex, the National Institutes of Health (NIH) now requires that sex be included...curricula. Corporations need to integrate these insights into product design. But eyes have been opened—and...