We narrowed to 398 results for: AGA

1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   ...resistance marker in the backbone. A selection on agar containing ampicillin will yield bacteria that have...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...mCherry expression using FACs sorting, confirming yet again that sgRNA structure and genomic recognition sites...

3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...

4. Hot Plasmids - May 2022

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   ...for variants that infect the CNS while selecting against those that also infect the liver.    Fig...

5. Antibodies 101: Isotypes

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   ...IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies. They...

6. Sharing is Caring: But How to Distribute Open Hardware?

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   ..., for example, can also be printed quickly in Madagascar. Platforms that allow for on-going creativity...

7. GPCRs: How Do They Work and How Do We Study Them?

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   ....1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of ...

8. Early Career Researcher Toolbox: Social Media for Scientists

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   ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...

9. Plasmids 101: Methylation and Restriction Enzymes

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   ...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...

10. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ... rules that I try to follow in my own research. Again, some of this may not apply or may not work for ...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...proteins inside a cell have a commercial antibody against them. There’s also a question about how specific...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted...

13. 3 Challenges in Plant Synthetic Biology

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    ...like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential, but...

14. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally...

15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...

16. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

17. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings...

19. Celebrating 15 Years of Scientific Sharing

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    Blog Post

    ...here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects...

20. Summer SciComm Series: Cognitive Load

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    ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...