We narrowed to 253 results for: CIL
-
TypeBlog Post...crossover to reduce ‘muddying’ of the signal and facilitate clear differentiation between multiple dyes during...
-
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...expressed a cytidine deaminase fusion with Cas9 with a uracil DNA glycosylase inhibitor with a degradation tag... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...pool of CRISPR software embodies solutions that facilitate most experimental applications in CRISPR engineering... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...expressed and a tracrRNA (the helper RNA that facilitate the processing of the crRNA) is also expressed... -
15 Hot Plasmids from 2017
TypeBlog Post...libraries The Gross Lab at UCSF recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...straight are to mark the upper left-hand corner with a pencil (not a pen — they bleed!), cut off the upper left-hand... -
Molecular Biology Reference
TypeGuide...Recommended Working Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol...Tetracycline 10 mg/mL 10 µg/mL *Carbenicillin can be used in place of ampicillin. DNA Sequencing for Plasmid...consists of Adenine, Cytosine, Guanine and Uracil (U). Uracil replaces thymine in RNA molecules. Every ...nucleotide bases as DNA, except thymine is replaced by uracil (U). Not all regions of DNA code for proteins. ... Addgene Resource(s) Cloning Plasmids Used to facilitate the cloning of DNA fragments. Cloning vectors...Nucleobase A Adenine C Cytosine G Guanine T Thymine U Uracil Single Letter Code: Ambiguous bases Nucleobase ... -
Lentiviral Vector Guide
TypeGuide...target species. Although chromatin availability facilitates integration, it does not explain the lentiviral...p75 ), a lentiviral tethering gene that helps facilitate integration. Despite knowing the general preference...transduction of lentiviral vectors in order to facilitate immunotherapy and gene therapy. Biomedicine &...unspliced and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid... -
Modular Cloning Guide
TypeGuide...or gene editing in filamentous fungi such as Penicillium or Aspergillus species. MoClo Yeast Secretion...sets of plasmids to generate constructs for Lactiplantibacillus plantarum and other species. Ubigate Collection... -
Sequencing Primers
TypeGuide... Forward Amp-R ATAATACCGCGCCACATAGC 5' end of ampicillin resistance gene Reverse AUG1 Forward CAATTTACATCTTTATTTATTAACG... -
Chemogenetics Guide
TypeGuide...Krashes, M. J., … Roth, B. L. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of... -
Plan Your Experiment
TypeGuide...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific ... -
Adenovirus Guide
TypeGuide...have matching left and right homology arms which facilitate homologous recombination of the transgene into...