...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light through direct... trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic toolbox has been...light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements include...

...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...

...first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared...

...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...

...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...

...kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...

...Milling and Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in ...system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that...

... to amplify the library, please refer to the depositor’s protocol for the best results. For some libraries...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources on our website...

...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...

...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...that Addgene uses for sequence verification of deposited plasmids....distribute primers. For sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone...of primers for sanger sequence verification of deposited plasmids. Below is a list of commonly used primers...

...selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification...

... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

...allowed us to handle and distribute such a large deposit. Once the project is complete, Ginkgo will have...-19. But, we can’t do this alone. If you have deposited plasmids at Addgene and would like to make them...

...scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ...

... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...