We narrowed to 658 results for: EPO;
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TypeBlog Post... tRNA suppression plasmids are available from depositing labs that have mastered this technique so it ...measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...
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Antibody Validation for Flow Cytometry
TypeBlog Post... is not efficient at the protein level at the timepoint studied Off-target activities of the RNAi Correctly... need to study protein expression at multiple timepoints. When the target of interest is critical to cell... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019... -
Delivery Methods for Generating iPSCs
TypeBlog Post...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC KLF4 LIN28... -
It’s CRISPR Week at Addgene!
TypeBlog Post...first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared... -
CRISPR 101 eBook...en español
TypeBlog Post...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ... -
Expanded Access to Rabies Plasmids
TypeBlog Post...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab. ... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours... -
DIY DNA Ladders from Penn State University
TypeBlog Post...kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID... -
Podcast: Interview with Addgene's Founders
TypeBlog Post...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...Milling and Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in ...system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that... -
What's New in CRISPR - May 2019
TypeBlog Post...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ... -
Why Do I Need an MTA Anyway?
TypeBlog Post...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take... -
What's New in CRISPR - December 2019
TypeBlog Post...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...allowed us to handle and distribute such a large deposit. Once the project is complete, Ginkgo will have...-19. But, we can’t do this alone. If you have deposited plasmids at Addgene and would like to make them... -
Working 9-5: an Addgene Co-op
TypeBlog Post...scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ... -
9 Tips to Achieve Success in Academia
TypeBlog Post...Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...