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Showing: 241 - 260 of 621 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... charged ions to rapidly pass through the cell membrane and initiate an action potential - a very useful...and close when exposed to light and change the membrane potential. OptoXRs are modified GPCRs. When exposed...downstream signaling pathways that can change both the membrane potential and a host of other cellular functions...Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proceedings of the National Academy of...

  4. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ....twitter.com/DWKWaPs0iY— Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon...pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by...pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford) December 3, 2021   Congratulations to all our winners,...

  5. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ... extensively with CRISPR, and recently became a member of the Addgene advisory board where he will help...and talk with us.  Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board with a...Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ...

  6. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...pic.twitter.com/X6QYXteRLT — Nika Heijmans (@nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats....co/MYMnXicWqb — Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re...pic.twitter.com/bDRqAKrWHz — Dyer Lab (@DyerLab_Emory) December 14, 2018 Thanks to all who participated in ...

  7. Genetic Code Expansion

    Type
    Collection
    ...concentration, and the cell lines used previously. Remember that the orthogonal pairs of synthetase and tRNA...

  8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...them. Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications...in the table): Basic functions: These functions embody the main goals of the software and should be the...genomes are denoted by organism name and genome assembly version. The names in the table appear as they...organisms and will help you whether you have a full assembly or not. It is an offline tool, but comes with ...

  9. Fostering communication in remote collaborations

    Type
    Blog Post
    ...meetings include all collaborators, from the lab members who are conducting the experiments to the PIs. ...closely replicates how you’d interact with team members in person, and improves overall communication.  ... informal communication you’d likely have with members of your lab that you see in person, and helps improve...

  10. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...This grows our relationship with our community members. We also find that, as a nonprofit, we attract ...is strength in diversity and in empowering team members to take initiative and give their best As we continue...Research Listen to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn ...

  11. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...an organelle-specific targeting signal or a transmembrane domain, biosensors can be targeted to a particular...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...providing structure and isotype determination, remember that it can do so much more than that!    References...

  13. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant...

  15. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may alter protein function...various loxP reporters. In both mouse and human embryonic stem cells, they found very high recombination...neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell ...

  17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...of CRISPR/Cas9." Cell Stem Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair...Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013).    ...

  18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...differentiation, the Zhang lab transduced human embryonic stem cells with the viral TF library, with each...lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were differentiated...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...

  19. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... out these questions ahead of time so that lab members have time to prepare their thoughts. Then at the...Set up a brief bi-weekly meeting where all lab members talk no science. No data presentation will appear...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ...

  20. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty...expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you'... running. Both Alex Chavez and John Doench are members of the Addgene Advisory Board. Additional Resources...
Showing: 241 - 260 of 621 results