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We narrowed to 619 results for: PROC

Showing: 241 - 260 of 619 results

  1. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...downstream from the Cas target. Conveniently, the process doesn’t cause double-stranded breaks and can deliver... CRISPR-Cas systems by Tn7-like transposons. Proceedings of the National Academy of Sciences of the United...

  2. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...mark it as a target for other immune cells, in a process known as opsonization. In antibody-dependent complement... the pathogen the antibodies are bound to in a process known as phagocytosis.  Other functions We’re still...

  3. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... agar slices.  Molecular biologists used this procedure daily before the advent of techniques like PCR...temperature (thus also saving freezer space).  The procedure becomes less attractive when noting that one step...

  4. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... also disrupted to elucidate their role in the process. The results of these studies led scientists to...sequence. Separate Cas proteins then express and process the CRISPR loci to generate RNA, which guide a ...

  5. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...samples and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting...Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36 (2014...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...and eukaryotes, which use a binary replication process whereby one cell copies itself and “splits” into...” genome with genes central to the replication process removed. This means the vector can deliver a genetic...

  8. Antibodies 101: Epitope Availability

    Type
    Blog Post
    .... Created withBioRender.com.   Biological Processes  If you’re feeling relieved that you’re working...visualize an abstract protein during your sample prep process, imagining how it starts and if any steps could...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...and how these arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  13. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...fragments containing the desired mutations. This same process can be used simultaneously for the generation of... multiple cloning steps. You can simplify this process by using NEBuilder which requires only a single-stranded...

  15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... tested in clinical trials. It is speeding the process of the discovery/development of genetic targets...off-target effects is a major limitation. The procedure will also need to be perfected until the efficiency...

  18. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... start studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature...home message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...material (such as plasmids) into mammalian cells, a process called transfection. Transfection is somewhat comparable.... Since the DNA introduced in the transfection process is not integrated into the nuclear genome, in the...

  20. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...quickly.  Through the years, we have perfected processes to curate the data our depositors share and enrich...and handle millions of queries. In an iterative process driven by our user experience experts, our software...
Showing: 241 - 260 of 619 results