We narrowed to 724 results for: Rel

...presence and explore glypican function, providing a reliable and accessible toolset for scientists worldwide...

...OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc...

...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...

... or live-cells, thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens in a new...

... choice due to their well-established methods, relative simplicity of production, and higher titers. However...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer/Shuttle plasmid ...which is how they got their name, but they are not related. AAVs require the presence of adenoviral genes ... inside of the adenoviral capsid and directly correlates to the size of the DNA removed from the viral...vector production platform, which like AdEasy™, relies on the homologous recombination of the shuttle ... preferentially infect certain tissues, which correlates with the specific diseases they cause. Ad5 and...

...apoptotic ablation of neurons through a process reliant on Flp recombinase. 5 Kazunari Miyamichi Genome...

... Puromycin selection. Kevin Brindle and Duncan Jodrell 17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression...

...synthesis via partial UAG codon reassignment and release factor 1 deletion . FEBS Letters , 586 (20), 3716...

...alleles in mouse embryonic stem cells. Return to top Related Addgene Plasmid Collections AAV Enhancer Collection...

...non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells...backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine...

...overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes them capable...transport: retrograde transport relies on dynein, while anterograde transport relies on kinesin. Different rates...diffusion across the synaptic cleft is usually severely impaired, and only few reports exist about dye...particle is taken up on one side of the cell and released again at another side of the cell after intracellular...visualize neuron connections that are functionally relevant, i.e. activity-dependent neuronal tracing. Apart...

...be functional in your host organism. Host Relevant Promoters Representative Empty Backbones Mammalian...

...AAV-CAG-hChR2-H134R-tdTomato CAG Activator Optogenetics Karel Svoboda 26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry ...

...genes makes them suitable to be packaged in the relatively small rAAV capsids. Learn more about optogenetics...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer/Shuttle plasmid ...which is how they got their name, but they are not related. AAVs require the presence of adenoviral genes ...available inside of the small AAV capsid and directly correlates to the size of the DNA removed from the viral...institution's best practices with regard to AAV-related research. See Addgene’s Biosafety Resource Guide...produce new viruses, eventually lysing the cell to release them. Optogenetics Biological technique to control...

...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis.... The HEK293T cells will produce the virus and release it into the supernatant. After an initial media...best practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide...

...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...compartment of action via sequestration. The strategy relies on several observations: Rapamycin is a membrane-permeable...physiological setting. Genetic techniques typically rely on overexpression or knock-down, where signaling...or small molecules and exceed a physiologically relevant exposure in intensity and/or duration. Both of...exposure to low-intensity blue light. This approach relies on the incorporation of light-oxygen-voltage (LOV... to this system by engineering cells that lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing...

...out there (and particularly CRISPR), it is now relatively easy to design constructs and repair templates...the other bits in order to achieve accurate and reliable multi-bit memory. To this end, Chris Voigt and...sites with no cross-talk. These pairs are closely related to the lambda phage integrase system which is the...intracellular pH are required in order to determine the relationships between pH changes and cellular events. To overcome...the oxidation of its substrate, luciferin, which releases energy as a photon. The photon energy is transferred...this interaction and thus triggers a synchronous release of the reporters which can then be tracked along...promoter-selection marker or promoter-inducible expression related vectors are also ready for further chaining. Plasmids...

...pMB1 (derivative) A Relaxed pBR322 ~15-20 pMB1 A Relaxed pET ~15-20 pBR322 A Relaxed pGEX ~15-20 pBR322... F1** A Relaxed pGEM ~300-500 pUC and F1** A Relaxed pCDF ~20-40 CloDF13 (CDF) D Relaxed This...pBR322 A Relaxed pColE1 ~15-20 ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed...replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies....Generally, control of replication is referred to as "relaxed" or "stringent" depending on whether the ori is...

...Salary –not relevant to most postdocs. Commercial successes in the performing arts- not relevant at all. ...opinions. The contents of this post should not be relied upon for legal advice in any particular circumstance...typographical errors.  No action should be taken in reliance on the information contained in this post and ...paths are not the only place to develop “industry related skills”. Many career paths in academia as well ...look broadly at opportunities to develop skills related to their areas of interest. Additionally, since...institutions of higher education nonprofit entities related to or affiliated with institutions of higher education...applicant works at institutions of higher education, a related or affiliated nonprofit entity, or nonprofit research...

...you are ready for us to release your plasmids, so we won’t immediately release preprint-associated materials... depositors had to email us with permission to release their plasmids for distribution, and we’ve found...status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly described ...described in a journal publication. Plasmids will be released 3 years after the deposit date. Don’t worry, we...we’ll let you know before these releases happen (and congratulate you on your paper!). And as always, .... But this way, as soon as we become aware the related journal article has been published we’ll get the...indefinitely. Of course, the fastest way for us to release your plasmids is still for you to notify us as ...