We narrowed to 556 results for: SEC

...2020). Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN PAMs with...double strand break.   Prime editors are the second generation of base modifiers available. This system...

...fluorescent reporter, the brain tissue was extracted, sectioned, and imaged, with all image sets available at ...information and includes annotated images of the brain sections describing the labeled regions, as well as the...

...long-read technologies. Short-read sequencing (“Second-generation sequencing”)  Short-read sequencing ... or complex sequences such as G-C rich areas, secondary structures, or repetitive regions. Short-read ...

... date with their third party libraries and any security updates. Most of the time, updating these libraries... which means there will be no further updates, security or otherwise. This might sound like Greek to you...

... google “how to write a scientific review.” The second thing I did was stare in horror at the limited ...paper. I also learned to appreciate the discussion sections of the primary literature as a resource that both...

...include surgery to remove cancerous masses (tumor resection), systemic chemotherapy to kill cancerous cells...added to the treatment recommendations for non-resectable mesothelioma by the National Comprehensive Cancer...

...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...

...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...

...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on its...

...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...

...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...

...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...

...of biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who ... I’ll highlight some of these questions in the sections below. Mentoring relationships can help identify...

... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...

...several applications of this technology. In each section below, we cite the original paper but link to plasmids...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...

...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...

... that we describe in the Materials and Methods section of our paper (1). Lastly, it is possible to build...sequencing as described in the Material and methods section of our paper (1). How might one troubleshoot GSRMs...