Hot Plasmids February 2024
Type
Blog Post
...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...
We narrowed to 332 results for: cloning
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10 Basic tips for mammalian cell culture
TypeBlog Post...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination vector with SapI... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly... -
CRISPR Plasmids - Yeast
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ... -
Distribution to Industry
TypeCollection... Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification... -
Worm Expression Resources
TypeCollection...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified... -
CRISPR Plasmids - Prime Edit
TypeCollection...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6... -
Hot Plasmids - August 2020
TypeBlog Post... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies... -
Lab to Office Culture Shock
TypeBlog Post... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...protocols and guides regarding pooled libraries, from cloning a pooled library to viral production to screening... -
Tips for arabidopsis transformation
TypeBlog Post...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium... -
AAVs in Retinal Gene Therapy
TypeBlog Post... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...