We narrowed to 329 results for: cloning
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TypeBlog Post...Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding...
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Hot Plasmids February 2024
TypeBlog Post...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005... -
10 Basic tips for mammalian cell culture
TypeBlog Post...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids... -
Plasmids 101: Degron Tags
TypeBlog Post... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...Type: Choose an empty backbone if you intend to clone your own insert, or use the other options to find... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination vector with SapI... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly... -
Tetracycline Inducible Expression
TypeCollection...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE... -
Synthetic Biology - Sensing and Signaling
TypeCollection...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation... -
Synthetic Biology - Strains
TypeCollection...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation... -
Synthetic Biology - Metabolism
TypeCollection...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation... -
CRISPR Plasmids - Bacteria
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ... -
Synthetic Biology - Networks and Gene Regulation
TypeCollection...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation... -
Zinc Finger Consortium Reagents
TypeCollection... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease... -
Hot Plasmids - August 2020
TypeBlog Post... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome...