We narrowed to 324 results for: cloning

Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab.

The RESOLUTE mission is to develop data and reagents to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most common oncogene in human cancer.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors.

Get started with Gibson Assembly Cloning!

Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more.

Use the mRNA expression FlowCode plasmids for barcoded expression of mRNAs of interest, for use in large-scale multiplexed in vivo mRNA expression screens.

This post highlights some of the tools Addgene makes available to industry scientists.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

Learn about the IBEX Knowledge-Base, which allows researchers to share validation data on imaging reagents for IBEX imaging.

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

Protocol - How to Ligate Plasmid DNA