We narrowed to 494 results for: control
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TypeBlog Post...a low multiplicity of infection (MOI). MOI is controlled by viral titer, so you’ll need to measure your...Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend determining the functional...
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CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...glycerol stocks, prepped DNA ready for our quality control processes - all of these samples have the 2D barcodes...along the way involves good aseptic technique and controls to ensure that nothing is contaminated. We use... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials are available... -
New Podcast Segment: Hot Plasmids
TypeBlog Post... Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...long term storage. Every preparation is quality controlled through next generation sequencing and spectrophotometry... -
Editor's Choice, July 2016
TypeBlog Post...example of a FRET experiment with all relevent controls near the end of the post also really helped put... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog Post...materials, repositories provide standardized quality-control and technical support to the community. Thanks ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...positive control in our knock-in experiments as it works quite efficiently and serves as a good control for...antibody.Note: Always include a positive and a negative control for the tagging experiment and test them in parallel... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...initiate our MTA process and perform standard quality control but will not make the plasmids available until:...Continue to Next Step". As part of our quality control process, we will sequence the full plasmid and ...received them and they will undergo our quality control process to verify your plasmids. Our friendly Addgene... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...Technology Austria, decided to take a novel approach to control for these effects, drawing on the rapidly growing...also available at Addgene and can be used as a control for your experiments. For more information, please... allowing labs to precisely and non-invasively control neuronal signaling. For more information on DREADDs...viral (AAV) system enables greater spatio-temporal control over expression and increases the number of species...Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., Nature. 2007... -
COVID-19 Resources
TypeCollection...available and more are currently undergoing quality control analysis in our lab and will be made available ... Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are available... -
CRISPR References and Information
TypeCollection... packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for... -
Podcast: Interview with Addgene's Founders
TypeBlog Post...nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's Viral Service - Why Virus? Why Now? ... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog Post...in generating Cas9 based tools to enable facile control of DNA and RNA. Special thanks to our sound... -
Fluorescent Protein Guide: Biosensors
TypeCollection... with different binding affinities or negative-control constructs used to characterize the sensor's behavior... GCaMP6f in PV cortical interneurons under the control of the E2 regulatory element Viral manipulation...in forebrain GABA-ergic interneurons under the control of the mDlx enhancer A viral strategy for targeting...Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849. Noboru Mizushima...expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective...molecular tension sensors reveal extension-based control of vinculin loading. eLife. 2018 Jul 19;7. pii:... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...combination of library coverage, mutational efficiency, control over where mutations are introduced, and scalability... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...that they form including genetic mechanisms that control who a female wants to mate with and if she decides... pan-arthropod genetic manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...1998). Regulatory elements within the ori also control copy number and stability, which influence the ...which consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be...