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We narrowed to 494 results for: control

Showing: 241 - 260 of 494 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038...

  5. Editor's Choice, July 2016

    Type
    Blog Post
    ...example of a FRET experiment with all relevent controls near the end of the post also really helped put...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...initiate our MTA process and perform standard quality control but will not make the plasmids available until:...Continue to Next Step". As part of our quality control process, we will sequence the full plasmid and ...received them and they will undergo our quality control process to verify your plasmids. Our friendly Addgene...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Technology Austria, decided to take a novel approach to control for these effects, drawing on the rapidly growing...also available at Addgene and can be used as a control for your experiments. For more information, please... allowing labs to precisely and non-invasively control neuronal signaling. For more information on DREADDs...viral (AAV) system enables greater spatio-temporal control over expression and increases the number of species...Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., Nature. 2007...

  13. CRISPR References and Information

    Type
    Collection
    ... packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... direct shipments weren’t able to clear import control. Partnering with a distributor allowed us to share... systems to allow for easier access to export-controlled items by streamlining permissions and paperwork...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...

  18. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...approach complete with freely available positive controls. Routinely test your cell line for mycoplasma ...Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector titration and...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...combination of library coverage, mutational efficiency, control over where mutations are introduced, and scalability...

  20. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...1998). Regulatory elements within the ori also control copy number and stability, which influence the ...which consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be...
Showing: 241 - 260 of 494 results