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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 328 results for: hal.1

Showing: 241 - 260 of 328 results

  2. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate - A Novel...practical terms, what GreenGate can do is “save you half a year,” in comparison to more tedious and time-...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...$47,476 and the date for implementation was set for Dec 1 2016. In order to be exempted from the need to pay... these institutions (populations potentially from 1 to 5761, according to the NSF data) and therefore ...what they are doing, however, may face greater challenges. Current salary information that we have been...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...activity to a transcriptional activation domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa... Stucka R, Hauber J, Lange UC (2016) Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes...University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ ...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while...INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade...DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this lethality to design...organisms, CRISPR-induced double stranded breaks are lethal because the non-homologous end-joining (NHEJ) repair...Cas9 will cleave the gene of interest, inducing a lethal DSB.     This system is distinct from those used...While the activation of transcription is a more challenging problem than repression, scientists have deposited...Streptomyces, yet genome engineering remains challenging for most organisms in the genus. Work from the...

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...native peptide bond at the split site. Figure 1: The base editor is split into two plasmids and co-transfected...each half with PhyB or PIF6, phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In ...identical in sequence to the unmodified enzyme. Each half of the base editor is fused to a fast-splicing split...induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer et al.,...

  8. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  10. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...products by their environmental impact on a scale of 1-10: the lower the score, the lower the environmental...wash your tips once and you cut your tips cost in half, not to mention the amount of plastic you’ve saved...

  11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...the output of the analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...together along with the Gibson assembly master mix for 1 hour, and then transform into competent cells. The...you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...

  13. A Control for All Seasons

    Type
    Blog Post
    ...compare the experimental group.     Figure 1: A, Most biological experiments will benefit from ... Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref,...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...

  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    .... Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research...since the Future of Research Symposia Ongoing challenges facing the scientific enterprise and the way ...landscape of the scientific enterprise will provide challenges and opportunities for us all.  For example, implementation...categorize all postdocs at institutions, will be challenging. At the same time, there is much opportunity ...the pressure on for further increases will be challenging, but will be aided by the development and implementation... is an important way to push the balance from challenge to opportunity. We hope to see you at the upcoming...

  15. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image used...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...

  16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a very big question...funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly politicized...., “The Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other...ramifications. Readers unfamiliar with academia’s challenges with publication and funding may be dismayed ...The three chapters in this section describe the challenges of trusting science in a “fake news and post-...describes the author’s thoughts on science’s biggest challenges over the coming years. It is arguably the most...the book? It doesn’t need to. The problems and challenges described in The State of Science have not changed...

  17. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, work...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely...absolutely equally, which means you only have one-half of the guilt. You can handle that, right? Big tip...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...

  18. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system...traditional FP fusions can also affect protein activity, half-life, or localization, these concerns are greater...

  19. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ... email in a busy inbox. Updated policy As of July 1, 2025, we are updating our Hold for Publication policy...choose this status for your plasmids. In fact, almost half of all new deposits start out with this hold! You...

  20. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ...split you got from the graduate student down the hall who got it from some technician downstairs who got...
Showing: 241 - 260 of 328 results