We narrowed to 291 results for: poly

1. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

2. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this...

4. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

5. Using Phosphoserine to Study Protein Phosphorylation

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   Blog Post

   ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

6. 10 Steps to a Perfect Science Talk

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   Blog Post

   ...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...

7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

8. Hot Plasmids: Spring 2025

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   Blog Post

   ...Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so...general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (...

9. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more...

10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...

11. Finding Your Perfect Job After University

    Type

    Blog Post

    ...and non-profit) you can also get a lot of office politics (many individuals trying to divert more resources...

12. Transferable Skills Guide: Teamwork

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    Blog Post

    ...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ...

13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ... Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction. In: Methods in Molecular Biology...

14. A Guide to Getting Started in Undergrad Research

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    ...dedicated to different career options from science policy to science communication and much more. I hope ...

15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post

    ...transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked target strand. The initial...

16. Trimmer Lab NeuroMab Collection

    Type

    Collection

    ...chain constant domain (C L ) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy ... depending on the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody ...

17. Using AAV for Neuronal Tracing

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    Blog Post

    ...restriction as well as controlled monosynaptic and polysynaptic spreading. AAV vectors cover some of these attributes...

18. Fluorescent Protein Guide: Biosensors

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    ...exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai...

19. Neurodegeneration Plasmid Collection

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    Collection

    ...pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 HTT GFP U6, EFS Huntington's Nicole Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1...

20. CRISPR Guide

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    Collection

    ...159 (3), 647–661. PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...