We narrowed to 290 results for: poly

...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

...expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this...

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...

...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

...Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so...general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (...

... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more...

...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...

...and non-profit) you can also get a lot of office politics (many individuals trying to divert more resources...

...chain constant domain (C L ) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy ... depending on the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody ...

...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ...

... Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction. In: Methods in Molecular Biology...

...dedicated to different career options from science policy to science communication and much more. I hope ...

...transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked target strand. The initial...

...restriction as well as controlled monosynaptic and polysynaptic spreading. AAV vectors cover some of these attributes...

...exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai...

...159 (3), 647–661. PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...

... DMEM complete + 10 µg/mL polybrene by diluting 20 µL of 10 mg/mL polybrene into 20 mL media. Thaw the...with DMEM complete + 10 µg/mL polybrene, the final concentration of polybrene in each well should be 10 µg...cells, resulting in a more homogenous (but still polyclonal) cell population. Depending on the transducibility...glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 ... the attachment of adherent cells) 0.45 μm polyethersulfone filter, Nalgene, 565-0010 (for viral preps...of the lentivirus in DMEM complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions...Lentivirus (μL) Volume of DMEM complete + 10 µg/mL polybrene (µL) 0 0 500 1:5 300 200 1:10 150 350 1:50 30 ...

...μg/mL polybrene. TIP: Polybrene increases the efficiency of viral infection. However, polybrene is toxic...drives RNA Polymerase III transcription for generation of shRNA transcripts. cPPT Central polypurine tract,...especially repeated Ts because polyT is a termination signal for RNA polymerase III. Note that these were ... for 12-15 hours. c. In polypropylene microfuge tubes (do NOT use polystyrene tubes), make a cocktail .... Hexadimethrine Bromide (Polybrene) Prepare a 1mg/mL solution of polybrene (Sigma-Aldrich catalog #H9268...to adjust the sequencing conditions if the DNA polymerase has difficulty reading through the secondary ...Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes VWR: #87003-290 Note: pLKO.1 could also...