We narrowed to 883 results for: prot

...polymerase (Pol) for replication and Gag structural protein on yet a third plasmid (packaging plasmid). Viral...viruses also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses...viral handling safety   Containment and personal protection are key to lab safety and the same principles...department if they are not currently provided. Eye protection or face shields are required whenever there’s...splash or spray risk in BSL-1. In BSL-2 labs, eye protection is required any time you have to work with BSL... cabinet. Many researchers choose to wear eye protection when they are working in a biosafety cabinet,...organization. It’s always okay to add another level of protection! When centrifuging viruses (a necessary step...

... they bind to a class of Argonaute proteins. The Argonaute proteins remove one strand of the siRNA and...from the Sánchez lab describes the plasmids and protocols required for both WISH and RNAi experiments, as...class” which provides more detailed materials and protocol resources. Find Sánchez lab Plasmids Visualizing...tissue. Once the riboprobes are created, the WISH protocol involves preparation of the tissue sample, several... the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered by feeding them to planarians...which planarian species one is working with, the protocol will slightly differ. For example, Girardia sp...riboprobes and dsRNAs into PR-T4P. The Sánchez lab protocol also provides step-by-step instructions for the...

... this system a modified TIR-1 protein can degrade degron-tagged proteins in an auxin dependent manner....a group discussion on the best strategies and protocols for different experiments.  In this blog post...structures, alternative polyadenylation for all protein coding mRNAs, and annotations extracted from other... discovered in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015)...scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL...each half to a gp41-1-N-intein. Split inteins are protein domains that naturally “associate, self-excise,...degradation (AID) system enables versatile conditional protein depletion in C. elegans." Development 142.24 (2015...

...the action depends on a surface glycoprotein – the only viral protein present on the virion surface – ...States while caring for a patient, despite wearing protective gear, and now comes news of a second Texas healthcare...does all of its damage with just seven structural proteins. But, as NPR explained, it’s not the virus itself...in PLoS Pathogens shows that the Ebola virus glycoprotein remains essential in allowing those infected...in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia School...Medicine have also recently described how the glycoprotein transforms itself to escape vesicles within ...reported in the Journal of Virology.  The EBOV glycoprotein’s role in infection also makes it a critical ...

... 2016). The protein capsid encapsulating the genome is majorly composed of three proteins that can be ...of terminal proteins capping the ends of linear double-stranded AdV genomes. Such protein-DNA structures...that AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed...along with a description of their experimental protocol. The three plasmids which have been deposited ...steatohepatitis cancer. In deficiencies of plasma proteins where Adv vector delivery is often used, the CRISPR...producer cells could produce corrective amounts of protein for any such disease. This technology therefore...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK....

...specificity, they purified HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish...applicability of base editing through protein engineering and protein delivery. Nature Communications, 8(...requires three elements. They are, broadly: A Cas protein (catalytically dead or nickase) fused to a deaminase...within the editing window specified by the Cas protein These elements were the starting point towards...efficiency of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9...formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor et al., 2017..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...

...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral...FRET reporters are comprised of two fluorescent proteins with very specific spectral properties that limit... and the orientation of the donor and acceptor proteins, which can be difficult to predict. Slower Dephosphorylation...single cells using translocation reporters. Nature protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids...biosensors Learn about using phosphoserine to study protein phosphorylation Additional Resources on Addgene.org...

...the methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color...detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We also highlighted reagent...features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope ...Without A Kit by Leah Schiesow We added to our protocol collection this year and along with it, wrote ...subset of these tools for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer...

...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression...the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can be generated...expressed in bacteria and extracted with common protein purification methods. Read on to learn more about...be better suited for the job. As single domain proteins, they can be expressed in bacteria, making nanobodies... the bench, this results in a shorter staining protocol because the primary antibody can be “labeled” ...super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879...

...PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences,...optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These resources can now be...Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin...used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang...and find plasmids containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for ...

...3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn’... and your lab? Organizing your lab notebooks, protocols, and to do lists can be easier than you think!..., and post-its to make finding your most used protocols & references easy to find It’s happened to all...can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular cloning... visibly labeled on the spine. For binders of protocols and data, consider using color coded binders and...your colleagues. Start small by identifying the protocols, guides, or references that your labmates ask ...history. Now you’ll be able to make updates to protocols in real time. You may also want to consider moving...

...Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to a protein of interest...leaf transformation: Check if there are working protocols available for your species This will save you ...first choice for establishing a new transformation protocol as it can easily show which tissue have been transformed...bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal...elucidate the subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons...started. Once you have established a well working protocol in your lab, it can become a strong tool for your...plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed...

...Caused by a mutation in the methyl-CpG binding protein 2 (MECP2) gene, Rett Syndrome is a rare, progressive...Bird lab found that only a portion of the MECP2 protein is necessary to rescue the neurological defects...Goldilocks” principle, in which too much of the MECP2 protein is as detrimental as too little, resulting in MECP2...researchers is how to deliver the precise amount of protein to each brain cell to restore physiological levels...reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease...: Schematic of the human MECP2 locus and MECP2 protein, showing mutations corrected by CRISPR/Cas9 in ...in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet 23:185–188 . https://doi.org/10.1038...

...length gene and/or protein is reconstituted. One way to reconstitute a split protein like Cas9 is to use...polypeptides that, when at the ends of two proteins, mediates protein trans-splicing, similar to an intron ...In search of a smaller, but equally potent Cas9 protein, the Zhang lab took a different approach. They ...toxicity. More AAV-based CRISPR systems Smaller Cas proteins SaCas9 isn't the only CRISPR enzyme that’s small...advantage that it can be turned off by anti-CRISPR proteins. The Sontheimer lab used NmeCas9 with AAV to edit...disease-causing loci in mouse liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers...papers yet published that deliver these Cas proteins with AAV, at just 1,108 and 986 amino acids, respectively...

...a programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo ...SpCas9 is not the only game in town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations...form of “bait” to identify new CRISPR-associated proteins within the bacterial genome. From this analysis...categories based on the architecture of the CRISPR protein in question: C2c1, C2c2 and C2c3 (short for Class...consistent with known roles for HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and...

...nuclease folds found in other proteins. The C-terminal domain (CTD) of the protein also belongs to the NUC ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...lobe—do not structurally resemble any other known proteins; they are completely unique to the CRISPR system... a single unpaired strand in the center of the protein. The repeat:anti-repeat duplex pokes out from the...RNA, confirming its sequence. This region of the protein varies between Cas9 enzymes from different species.... The stem loops bind along the outside of the protein, spanning both lobes. Stem loop 1, nestled between...shows the guide RNA stabilizing the DNA-binding protein portion of the CTD, ready to check for PAM sequencines...

...sequence using all six open reading frames UniProt: A database of protein sequence and functional information...Biology Reference Find molecular biology protocols Browse our protocol video collection ... of Blue Flame plasmid pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze...with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database ExPASy translation...IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics ...needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track your experiments...

...in hosts carrying the bacteriophage lambda pir protein. This allows conjugation between a pir-containing...might be useful in a broad array of pathogenic Proteobacteria. We placed the toxin genes under control of...that encode one of two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system...PMC3936740.  Giacalone, Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...Exchange Vectors for the Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology...et al. "Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology." Journal of...

...dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions with ...DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent protein to the ...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular... in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...

...RNA. Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium...reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more! Background...: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding...Thermus thermophilus (TtAgo), showing that this protein could cleave DNA in vitro, directed by phosphorylated...loading appears limited to the period when the protein is being synthesized, so NgAgo remains faithful...