We narrowed to 303 results for: rigi

1. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   Blog Post

   ...Additionally, the TAM changes based on the species of origin of the TnpB protein, much like how PAM sites change...

2. QC Sequencing Technologies at Addgene

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   Blog Post

   ...relies on sequencing by synthesis, but modifies the original Sanger procedure to improve throughput by increasing...

3. Plasmids 101: Shuttle Vectors

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   Blog Post

   ...population. A bacterial chromosome has a single origin of replication (ori), where DNA replication begins...

4. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   ... (AAG). When adenine base editors (ABEs) were originally being developed for A → G edits, researchers ...

5. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ...Bacterial Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design...

6. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody...

7. 5 Reasons to Use Reddit for Science Communication

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   Blog Post

   ...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...

8. Tips for CRISPR Gene Editing in Mice

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   Blog Post

   ... with RNAs encoding Cas9 into the oocyte. This original method was developed as the use of CRISPR/Cas9...

9. Overcoming the AAV Size Limitation for CRISPR Delivery

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   Blog Post

   ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...

10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

11. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...

12. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...you don’t get the specific position he or she originally called about, you can keep in touch and reach...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post

    ...ideal system to observe embryogenesis live. The origins, divisions, and movements of each of the thousands...

14. Tips for Writing a Good Cover Letter

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    Blog Post

    ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...

15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...arabinose-inducible promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide ...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  ...

17. Bioinformatics at Addgene

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    Blog Post

    ...sequences each week. Our internal software was originally designed to help us work in large formats, such...

18. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...

19. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ... such as the antibiotic resistance gene and the origin of replication are necessary to replicate plasmids...

20. The Advances Behind The World's First Personalized CRISPR Treatment

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    Blog Post

    ...needed! Minimizing off-target editing ABEs were originally developed from RNA deaminases, using directed...