We narrowed to 323 results for: sars

1. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming from above”, which is when other...

2. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep 5 ...

3. 3D Printing Meets CRISPR Cas9

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   ...appreciate the 3D structure of these proteins, it is necessary to download a pdb file of the structure’s atomic...

4. Cloning Mammalian Cells with the Agarose Method

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   ...agarose overlay does have one tricky step. It is necessary to pour the agarose onto the recipient plate at...

5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...situations where maintaining heterozygosity is necessary and genetic recombination can potentially cause...

6. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...cancer cells, attacking the cellular machinery necessary for division. This approach also kills dividing...

7. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene performs...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... derived from GFP, i.e. the red FPs, are not necessarily monomeric, even if they were described as monomeric...

9. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...GFP-Puro cassette. Three double stranded breaks are necessary for knock-in: one on either side of the GFP-Puro...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    .... Packaging and Envelope plasmids provide the necessary components to make lentiviral particles. Stable...

11. Genome-wide Screening Using CRISPR

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    ...question and experimental system are absolutely necessary to ensure that you select the correct CRISPR library...

12. Plasmids 101: Multicistronic Vectors

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    ...the clever experiments and innovative solutions necessary for advancing the field. References: 1. Internal...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...will not need to ‘domesticate’ all the basic and necessary elements in order to start using the MoClo system...

14. Antibodies 101: Producing Recombinant Antibodies

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    ...A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate, albeit at relatively...

16. MXS Chaining

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    ...recognition sites. Thus, chaining methods are not necessarily suitable for combining endogenous sequences. ...

17. Simplify Cloning with in vivo Assembly

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    ...perform modifications, simply by adding all the necessary primers to the same PCR mix. Pairs of primers ...

18. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...side of the membrane, then this step may not be necessary. In fact, you may get cleaner results by not permeabilizing...

19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...and multiple promoters were used to express the necessary components in E. coli (Klompe et al., 2019). Recently...

20. Lentiviral Vector Uses and Overview

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    ...increasing the number of recombination events necessary to create a replication-competent virus. Second...