We narrowed to 13 results for: sars
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TypeProtocol...plan to phosphatase treat your cut vector it is necessary to use 5'-phosphorylated oligos. This is an option... of 50μL - add additional annealing buffer if necessary to get to 50μL. Efficient annealing can be achieved...
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Protocol - How to Run an Agarose Gel
TypeProtocol...over. Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close... -
Kit Free RNA Extraction
TypeProtocol...Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar... -
Protocol - How to Design Primers
TypeProtocol...Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction. One needs to design... -
Weighing Reagents Protocol
TypeProtocol...scoopula or spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop... -
Water Bath Protocol
TypeProtocol...way, as items are floating, you do not need to necessarily maneuver the bottles or tubes to identify them... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid DNA for experimental... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... any spills immediately, and decontaminate as necessary. For large spills that cannot be easily cleaned... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...into a 10μL reaction, scale the reaction size as necessary - being sure to increase the amount of buffer ... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol... density gradient media, it is reportedly not necessary to remove iodixanol prior to use of your purified... -
Pouring LB Agar Plates
TypeProtocol... in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling over ... -
CRISPR Library Amplification
TypeProtocol...genome, for example. Amplification is usually necessary to produce sufficient quantities of library for... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol... Calculate the amount of Fugene® and OPTI-MEM necessary given that each reaction will require 6 μL FuGENE...