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We narrowed to 602 results for: she

Showing: 241 - 260 of 602 results

  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with...including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows the user ...Restriction Enzyme Tool by Promega Material Safety Data Sheet by RSS-ICTD Amino Acid Structure Quiz/Card by Andrey...

  2. Antibodies 101: ChIP

    Type
    Blog Post
    ...Sonication provides a truly random digestion, but is harsher and requires more optimization. Enzymatic digestion...more of the protein:DNA complexes. Once you’ve finished fragmenting the DNA, spin down the samples to ... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... question. Three different labs have recently published work that provides a solution to this problem....genomic deletions In PRIME-del, developed by Jay Shendure’s Lab, paired pegRNAs work with the PE2 enzyme ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

  5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions... is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop CRISPR-based...Feinberg A, Vogelstein B (1983) Hypomethylation distinguishes genes of some human cancers from their normal...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

  8. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ... to generate high-fidelity reactions. (Need a refresher? The five rules are: (1) don’t use the same overhang...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    ...those tips for you in one place! First, a quick refresher: prime editing is a “search and replace” genome...j.cell.2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    ...easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched / developed...insight into an area - not just working within the established network of your peers. Furthermore, using new...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number Variation...10.1007/978-1-4939-7778-9_9. PMID: 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

  12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...where they are physically located. DNA is then sheared into pieces small enough to be pulled down via ...

  13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from...uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin Hsu Harvard University, and David... the Broad Institute and Harvard University. Max Shen is a Ph.D. candidate at MIT. His research uses applied...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    ...removed to leave you with DNA. This DNA is then washed, and either eluted from a fixed surface or precipitated...glass particles are pelleted by centrifugation and washed before DNA is eluted with water or TE. The Bishop...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...its various derivatives, biological samples are enmeshed in a gel of a highly absorbent material that is...full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity can...20210110. https://doi.org/10.1098/rsta.2021.0110  Sherry DM, Stiles MA (2022) Improved fluorescent signal...

  17. MXS Chaining

    Type
    Blog Post
    ...High-throughput cloning, in a nutshell, is the systematic combination of different genetic sequences ...of coding sequences Sladitschek and Neveu, 2015; Shetty et al., 2008; Anderson et al., 2010 Among the...1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...can be eluted once the other materials have been washed away (Figure 1).      Figure 1: The steps...while the media is aspirated. The beads are then washed, followed by antibody elution. Whatever method ... strong ones, consider increasing the number of washes performed during your harvest. Validation Recombinant...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014...
Showing: 241 - 260 of 602 results