...potential to overcome some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...(non-LTR TEs). LTR retrotransposons are like retroviruses both in their structure and mechanism of replication... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...

...-villain geneticist named Zobrist who designs a virus that can potentially kill one-third of the human...

...and information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide...

...screen identifies genes essential for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673...

...Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

...out there for extracting DNA from tissues, blood, virus, or other samples, for PCR and gel cleanup, and ...

...in vitro. I transduced the cultured cells with a virus that encodes a transgenic protein for the HEK 293T...

...Colorado, Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304 Luciferase-V5 blast...

...C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B. taurus ) Library Size Genome-wide...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

...2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID...

... of Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus (CMV, PX601 [#61591] ) or liver-specific tyroxine...

...E, Levi BP, Ting JT, Zeng H, Tasic B. Enhancer viruses for combinatorial cell-subclass-specific labeling...

...responses of the immune system upon infection with virus and, like other cytokines, enable communication ...TCF16 CR2 complement component (3d/Epstein Barr virus) receptor 2 C3DR, CD21, SLEB9 FCGR2B Fc fragment... 1 (Xenopus laevis) DKK-1, SK EBI3 Epstein-Barr virus induced 3 IL27B EDN1 endothelin 1 ET1, HDLCQ7 EDN2...fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog) HBGF...receptor GPR38, MTLR1 MPL myeloproliferative leukemia virus oncogene C-MPL, CD110, MPLV, TPOR MSTN myostatin...described. Symbol Name Synonyms EBI3 Epstein-Barr Virus Induced 3 IL35B, IL27B, IL35 subunit IL10RA interleukin...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... be efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9...editing method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the...H840A nickase and M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) for "search and replace" ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

...guarantee viral service, but lets us know what viruses would be helpful to the scientific community. Penn...

...11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 pBS500 ...