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We narrowed to 469 results for: Lif

Showing: 261 - 280 of 469 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...genomes into hosts in order to continue their lifecycle and replicate. This feature can be harnessed for...for stem cell therapies, which rely on slowly proliferating cell populations that can be difficult to transduce...Lentiviral Plasmid FAQs Answered The Retroviral Lifecycle More Viral Vectors 101 topics More Lentiviral ...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...included versions with gentamicin-resistance (amplified from TP997). We validated these vectors, which...system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...10.1038/35888  Iwakawa, H., & Tomari, Y. (2021). Life of RISC: Formation, action, and degradation of RNA-induced...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...figure out all the ways a given ELN can make your lab life easier. If you can talk to an ELN support team directly...more tools for reproducibility 5 great apps for lab life Developing Lab Management Software for Biology ...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...from Gary Yellen’s lab is LiLac, a fluorescence lifetime sensor for lactate developed using a high-throughput... for performance under two-photon fluorescence lifetime imaging. B) LiLac responses are highly resistant...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... can virtually eliminate plasma HIV-1, improving life expectancy and quality for HIV-1 patients. However...along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled samples showed...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...of significance? You’ve already done the heavy lifting, so don’t let the statistical analysis of your ...that matter. However, this is oftentimes an oversimplification. If your independent variables are affecting...

  11. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...students chose to go the solo route for their Major Qualifying Project (or MQP, the school’s equivalent of a...around 10th grade, I snapped out of the phase of my life that had me listening to Simon & Garfunkel’s “I ...

  12. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...certain tumors. However, antibody stability, half-life, tumor penetration, inadequate concentration, mutation...therapy for prostate cancer and is aimed at increasing life expectancy by 4.1 months as opposed to curing the...

  13. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...genomes into hosts in order to continue their lifecycle and replicate. This feature can be harnessed for...Gamma-retroviral Packaging Systems The Retroviral Lifecycle Types of Viruses Viral Vector Elements More Viral...

  14. Adenovirus Guide

    Type
    Guide
    ...also resulted in a reduction of viral vector amplification and overall lower viral titers. Because of this...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Densmore’s lab at Boston University have greatly simplified the process of generating multiple E. coli gene...cellular processes such as differentiation, proliferation, and apoptosis. In humans, mutations that permanently...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...learned in the lab that will hopefully make your life a little easier. These can range from technical ...
Showing: 261 - 280 of 469 results