We narrowed to 753 results for: ELP

1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...happening fast and we’re glad to join these efforts and help academic and industry scientists through plasmid...

2. Hot Plasmids and Viral Preps - March 2021

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   ...contributed a set of plasmids to the repository that will help epigenetic researchers custom design their own engineering...

3. Hot Plasmids and Viral Preps - January 2021

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   ...such as fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify...

4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ... or transcription start site function would also help to reveal the impacts of a lncRNA on nearby genes...

5. Fluorescent Proteins 101: Introduction to FRET

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   ...detected even if the two proteins form a complex. To help troubleshoot your experimental setup as a potential...

6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ... between virions and the cell surface, and also helps form DNA and retroviral-particle or VLPs complexes...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...reduces variability in genotype and phenotype and helps improve the reproducibility of experiments. Inbred...

8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...SunTag, and VPR (Zhou et al., 2018). We hope this helps you chose a CRISPR activator for your experiment...

9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...small protein inhibitors of CRISPR-Cas systems that help phages evade the CRISPR-Cas immune system of bacteria...

10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...   Strategy #4 (ProD): Create promoters with the help of epigenetic analysis and designed using stretches...

11. Viral Vectors 101: Calcium Sensors

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    ...along with a little nuance discussed below, will help match your experiment with a sensor that is tuned...

12. Viral Vectors 101: Virus Safety

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    ... working with before you work with it. This will help you understand what safety precautions to take, ...

13. Antibodies 101: Choosing the Right Isotype

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    ...explained when and why antibody isotypes matter and helps you choose the right antibody for your application...

14. Plasmids 101: Modular Cloning

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    Blog Post

    ...cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz’s...

15. Progress Towards a PAM-Free CRISPR

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    ...isn’t always ideal. But the increased flexibility helps even when it’s not an ideal site: moving several...

16. Engaging with science and society at pgEd

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    ...preparing you for future work in these areas, and in helping you decide whether you enjoy this kind of work....

17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

    ...define productive routes for advocacy efforts and help participants develop the efficacy of their advocacy...

18. 5 Tips for Troubleshooting Viral Transductions

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    ...transduced. Here, I will share five hail Marys that have helped me troubleshoot some technical obstacles when working...

19. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...transcription due to the presence of additional helper sequences (2-3). As alluded to above, termination...

20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...to attend and speak about Addgene’s experience helping scientists share plasmids, especially those of ...

21. The Materials Science of Optogenetics Experiments

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    ...polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light. Test your...

22. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...regional, and national levels. These skills will also help the person mentor others more successfully. But ...

23. Experimenting in an Open Source lab: from CRISPR to Cats

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    ... lab, we have members with a wealth of skills to help do science on the cheap using equipment that would...

24. How-to: 5 Steps to a Great Panel Discussion

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    ...time. Both the moderator and program organizers can help make sure the event stays on schedule. People can...

25. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped along with very little...

26. Plasmids 101: Protein Expression

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    ...signaling and communication. She loves being able to help the scientific community share plasmids.   Additional...

27. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ...pegRNA design with PRIDICT/PRIDICT2.0, ePRIDICT can help you understand how chromatin might influence editing...

28. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...respectively, are selected (6). These previous blog posts help with the decision of which FP you should use and...

29. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...scientists––CrispyCrunch! CrispyCrunch is a web app that helps scientists design and analyze batches of CRISPR ...

30. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...cytometry, there are many additional resources that can help ensure that your first spectral flow cytometry experiment...

31. Of Myc and Men

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    ...Evan, et al. hoped that their new monoclonals would help the field explore Myc’s role in cell proliferation...

32. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...fluorescent protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies...

33. Addgene's Cancer Collection Pages Connect the Research Community

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    ..., and Addgene is accelerating this research by helping to make new cancer resources easily accessible ...

34. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    .... EGFP and mCherry). These simple strategies can help you make better fluorescent fusion protein reporters...

35. Genome-wide Screening Using CRISPR

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    ...design and validation practices, CRISPR libraries can help you learn a lot about your phenotype of interest...

36. Which Fluorescent Protein Should I Use?

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    ...the greenfluorescentblog.wordpress.com.   More Helpful Websites & Resources: FP guide at Addgene Interactive...

37. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...approaches you with glowing feedback from a client you helped. Maybe you awkwardly say thanks and move on with...

38. Great Results Start with Great Standard Curves

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    ...its absorbance is plotted on the y-axis. With the help of graphing software, you then generate a trendline...

39. Antibodies 101: Producing Recombinant Antibodies

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    ... supernatant for further processing. It may be helpful to incorporate regular feeds during this incubation...

40. Simplify Cloning with in vivo Assembly

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    ...sequence manager, such as SnapGene, can be very helpful to confirm primer design.  Use minimal template...