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  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  3. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

  6. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...to your community. For those of you who have the desire and ability to, some communities are looking for... results. Also consider using this framework to design your future experiments.  Use Addgene’s AAV Data...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

  11. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

  12. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

  13. K. phaffii: Rising to the Occasion

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    Blog Post
    ...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  14. Hot Plasmids - January 2023

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    Blog Post
    ...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce...they really want to do is get the paper off their desk and move on. Nevertheless, editors can and do play...

  16. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  17. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  18. AAV Viral Preps

    Type
    Collection
    ...comprehensive high-quality quality control process includes titering, purity assessment, sterility testing...

  21. Plasmids 101: Inducible Promoters

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    Blog Post
    ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...yellow flame light-regulated two component systems designed by the Tabor lab. Which inducible promoter is ...

  22. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...control, generates  educational materials, and provides viral vector technical service (details about ...tracking. Our Software and IT Teams made major upgrades to our inventory management systems to enable ...

  24. AAV Q&A with Tim Miles

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    Blog Post
    ...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

  25. Antibodies 101: Isotypes

    Type
    Blog Post
    ...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

  27. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

  28. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

  29. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

  30. Viral Vectors 101: Viral Applications

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    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

  31. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency...

  32. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

  34. Plasmid Collections

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    Collection
    ...together based on type or application. Browse our guides to find the plasmids and science information you...

  35. AAV Molecular Tools

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    Collection
    ...Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation...

  37. RANbodies: Reporter Nanobody Fusions

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    Blog Post
    ...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

  38. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1...create other photoswitchable kinases? The general design of psMEK1 also worked for creating three other ...

  43. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...most popular website in the world. I know, right? Upgrades to the internal LIMS pages and excellent work ...

  44. Summer SciComm Series: Cognitive Load

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    Blog Post
    ... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  46. Addgene: The First Twenty Years

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    Blog Post
    ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request...

  47. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

  48. Plasmids 101: Cre-lox

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    Blog Post
    ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...
Showing: 651 - 700 of 983 results