We narrowed to 933 results for: KIN

...selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic...photocurrents, and is more selective for K+ than HcKCR2, making it more suitable for in vivo optogenetic use.   ...

...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared..., Miller, S. M., Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, ...

...her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

...medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus...be able to support diverse groups of scientists working in a wide range of research fields and geographical...

... to improve our resources for this community. Networking with microbiologists Over the course of the conference... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

... be. Managers must ensure that their teams are working well together and collaborating effectively with...ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue, pointing out...

...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ... they are at risk of being fired and should be looking for a new position. One of the most common mistakes...

...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C...

...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...

... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great...

...484 variants, including some potential tetramer-breaking mutations. The library was then screened through...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with...

...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the...

... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from...

...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story.  At this point you may be thinking  “I don’t want to bury my results all the way ...

...catalog, so that you will know exactly what you are working with. We will also be collecting feedback from ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ...

... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...

... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition...

...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also...

...cell lineage. Variations of this library allow tracking of transcription or chromatin accessibility at... town! Pooled libraries can be designed for all kinds of different high-throughput experiments. Our most...

...properties (for example, overall brightness or on/off kinetics) that makes them suitable for different applications...with a CAG promoter exhibits high specificity for Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c...

...sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ...

...you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist...really good time! If it sounds right for you, start thinking outside of your current research box, send out...

...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...

...Incredibly imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management...

...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited...

...interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use...

...and how it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system...negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559...

...effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of those off-target...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...

.... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...

...differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic...mimic the human lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating...

...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...

...Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A ...

...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award...

...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...

...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...

...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page...passed down through cell division. You can find all kinds of different lentiviral and AAV plasmids as well...

...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

... noise in the process of sampling and inference-making. Even perfect transparent and rigorous studies ...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest ...

...box above. The number of action potential (AP) spiking events are indicated below the trace; asterisk ... now available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo...

... light Chemical reaction High-energy photons Kinetics of photon generation Slower Faster Cofactors...and more. These variant proteins offer different kinetics, substrate requirements, and photon emission wavelengths...

...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

...proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...

... those first. Depending on the markers you are looking at and the antibodies you are using, you may need... general consensus seems to be that if you are looking for a rare population or are unsure what amount...

...however can interfere with other genetic pathways making it difficult to link a phenotype to the gene being...TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment...

...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...

... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...