Addgene

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Showing: 651 - 700 of 721 results

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Experimenting in an Open Source lab: from CRISPR to Cats

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Blog Post

... offers members and the public cheap access to personal manufacturing tools – a 3D printer, a laser cutter...
Hot Plasmids: Winter 2025

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...plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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...Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu...
Polymerase Chain Reaction Overview and Applications

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...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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...Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy...
Academic vs. Industry Postdocs

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...is not always the case. There are “difficult personalities” in every work setting and no manager will ever...
Cloning Mammalian Cells with the Agarose Method

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...cloning. Taking a page from bacterial cloning, we reasoned that we should be able to keep cells moist and...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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...Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

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...forward the next year when both the Yamanaka and Thomson labs showed the successful generation of human ...
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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... efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, it demonstrates significant...
Addgene's Cancer Collection Pages Connect the Research Community

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...Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ...
Plasmids 101: Optimizing Plasmid Yields

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...of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields...
Genome-wide Screening Using CRISPR

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...Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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...accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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...talking to people and representing yourself as a real person, so that students see academics as well-rounded...
Tips for Using BLAST to Verify Plasmids

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... Jason Niehaus...
Which Fluorescent Protein Should I Use?

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...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...
Great Results Start with Great Standard Curves

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... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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...Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...
CRISPR 101: RNA Editing with Cas13

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...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...
Getting to Know Expansion Microscopy

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.../10.1126/science.1260088 Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue...
AAVs in Retinal Gene Therapy

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...gene therapy revolution and the development of personalized medicine. References and further reading 1....
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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...single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average...
Plasmids 101: Colony PCR

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...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org ...
Tips for Screening with Yeast Two Hybrid Systems

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...43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...
Plasmids 101: Protein tags

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... Additional resources: Young CL, Britton ZT, Robinson AS, Biotechnology Journal 2012 , (7): 620-634....
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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...boring. I think that such tendency is one of the reasons why we have only three biology-related Nobel prizes...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ...
CRISPR 101: Any Base Transversion Editors

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... Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion editors...
Twenty Years of Sharing: Expanding Accessibility

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... Rachel Leeson...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...
Plasmids 101: Plasmid Incompatibility

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...plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of ...
An Inside Look into Addgene's Dev Team

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... area, and when something urgent comes up, that person is the first in line to resolve it for expediency...
Bioinformatics at Addgene

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... Jason Niehaus...
CRISPR-based Genome Editing Systems For Editing in E. coli

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...2020. His research focuses on the efficiency of transposon-encoded CRISPR/Cas systems in bacteria, specifically...
Deciphering the Mysteries of Behavior with Viral Vectors

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...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...
Designing Your Chalk Talk for the Academic Job Interview

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...compatible functional livers.” Why are you the best person to do this research? Do you have innovative tools...
Analyzing CRISPR Editing Results with ICE from Synthego

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...computed during generation of the ICE Score, the Pearson correlation coefficient (r) is also computed and...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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...Road Ahead." Journal of Alzheimer's disease & Parkinsonism 6.5 (2016). PubMed PMID: 27882265. PubMed Central...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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... 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

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...the ability to cleave the RNA target. Table 1: Comparison on Common CRISPR Enzymes Name Enzymatic Domains...
Using Video to Share Your Science: We Share Science

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...me. I read a variety of psychology, business, personnel, and sociology journals when I get a chance, but...
Changing Labor Laws Bring Increased Postdoc Wages

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...getting outside academia. There is also a valuable lesson for the enterprise: the time for talk is over and...
Five Popular Model Organisms, Part 2

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...research (Ellenbroek and Youn, 2016). For these reasons rats were once the most used mammalian model in...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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..., Kosik, K. S., Arias, C., Acosta-Alvear, D., & Wilson, M. Z. (2020). A Scalable, Easy-to-Deploy, Protocol...
Quick Guide to Near-Infrared Fluorescent Proteins

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...Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of...
Filming Science Videos in the Age of Social Distancing

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... outside at a distance, or film inside with one person in a room. I wouldn’t be in there with them. I ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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...Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted...
Fluorescent Proteins 101: When GFP lets you down

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...sequence of the protein of interest. One of the reasons that this works well is that the N- and C-terminus...

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