We narrowed to 398 results for: AGA

1. Addgene: The First Twenty Years

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   Blog Post

   ...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...

3. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments...

4. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

5. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

6. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

7. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   ...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

9. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...the parent cell population using the library, propagated it, separated it into roughly identical replicate...

10. Working with Nuclear Receptors

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    Blog Post

    ...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...

11. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

12. Hot Plasmids February 2024

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    Blog Post

    ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

14. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,...

15. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

16. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...

17. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

18. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

19. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

20. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    ...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...